PCR primers for bisulfite sequencing (5′ to 3′), from the cyclin D1 promoter region, 1 kb upstream, and 3 kb upstream
Location . | Primer . | Temperature, °C . |
|---|---|---|
| D1 promoter | ||
| US1 | TTTTTTTAGTTGTTTTTTATTGTAGA | 52 |
| DS1 | CACAAAAACTAATATTCCATAACT | 52 |
| US2 | GGGTTTTTTGTATTTTTTTTTTTTGG | 54 |
| DS2 | AAACAACTAAAAAAAACTATAAATCCT | 54 |
| 1 kb upstream of D1 promoter | ||
| US1 | TAGGGTAAATTTTAAAGGTGAA | 54 |
| DS1 | AAAAAAAAAAAATACAAAAAACCC | 54 |
| US2 | TGTTTTAGGTAGAGGGGATTAA | 58 |
| DS2 | AAAAAATAAAATAAAAATAACTCTACA | 58 |
| 3 kb upstream of D1 promoter | ||
| US1 | TTAAGGGTTTAATAATGGAAAA | 53 |
| DS1 | ACAAAAAACAAATAACCCAATC | 53 |
| US2 | GTTGTAGGTGATTTTATTTGGG | 57 |
| DS2 | CCAAAATTAACTAAAATCACCTTC | 57 |
Location . | Primer . | Temperature, °C . |
|---|---|---|
| D1 promoter | ||
| US1 | TTTTTTTAGTTGTTTTTTATTGTAGA | 52 |
| DS1 | CACAAAAACTAATATTCCATAACT | 52 |
| US2 | GGGTTTTTTGTATTTTTTTTTTTTGG | 54 |
| DS2 | AAACAACTAAAAAAAACTATAAATCCT | 54 |
| 1 kb upstream of D1 promoter | ||
| US1 | TAGGGTAAATTTTAAAGGTGAA | 54 |
| DS1 | AAAAAAAAAAAATACAAAAAACCC | 54 |
| US2 | TGTTTTAGGTAGAGGGGATTAA | 58 |
| DS2 | AAAAAATAAAATAAAAATAACTCTACA | 58 |
| 3 kb upstream of D1 promoter | ||
| US1 | TTAAGGGTTTAATAATGGAAAA | 53 |
| DS1 | ACAAAAAACAAATAACCCAATC | 53 |
| US2 | GTTGTAGGTGATTTTATTTGGG | 57 |
| DS2 | CCAAAATTAACTAAAATCACCTTC | 57 |