Table 2. PCR primers for ChIP assays... | American Society of Hematology

Table 2.

PCR primers for ChIP assays

Name	Forward	Reverse	Temperature, °C
GAPDH	TAGTGTCCTGCTGCCCACAGTCCAG	GGCGACGCAAAAGAAGATGC	68
β-globin 5′ HS2	TGAGTGGCAGTGTTTAAAGGG	CCATCCCTGATGAGTTTTTCCTC	63
MYEOV	CTACCCTCTCCTCTTCCTTCTC	TACGCATGGCAGACATCAC	60
MTC	AGATTAAACTGCGTCTTGTTCG	TAGAGGCTTTGTCCTACCATCC	62
P519	ACATGAGCGATCTGGTAAGGACTC	CCACATGAGAGCTCACTCTGCAA	60
P519_bkp	GAGGGAGACCTATAACATGAGC	TACTGTAAGGGAGATGTGTTGG	62
UP10K	TACAGACCATCACCGTTGCC	TTCACTCACCACCCATCCTC	65
UP3K	TTTCTCCTGACCGACCATCC	CACTGTCTTCTTAAAACCCACC	63
D1 promoter	CCTTGGGCATTTGCAACGAC	CGCATTTCCAAGAACGCCAC	64
D1 intron 1	TTCTTTGCTACTCACCCCC	CCCTTCCTCCTTCAGAAAATAC	61
D1 intron 4	TGGCTCAGAAACACCATCG	GGACATTCCACCCACCAAAG	65
Down 60 K	ACAATATCCCACCATCTCCTC	AACCCTAACACTCCCCATC	61
Eμ	CAGCCCTTGTTAATGGACTT	GGAAAGTTAAATGGGAGTGACC	65
3′ Cα HS4	TCCAGTCTGAAAAACAAGACC	ACCTCCCCCAATGCAAATC	63
3′ Cα HS3	AGGTCTCGACTTAGCACTG	GGCATGTTTCTCAGAACAGC	66

Name	Forward	Reverse	Temperature, °C
GAPDH	TAGTGTCCTGCTGCCCACAGTCCAG	GGCGACGCAAAAGAAGATGC	68
β-globin 5′ HS2	TGAGTGGCAGTGTTTAAAGGG	CCATCCCTGATGAGTTTTTCCTC	63
MYEOV	CTACCCTCTCCTCTTCCTTCTC	TACGCATGGCAGACATCAC	60
MTC	AGATTAAACTGCGTCTTGTTCG	TAGAGGCTTTGTCCTACCATCC	62
P519	ACATGAGCGATCTGGTAAGGACTC	CCACATGAGAGCTCACTCTGCAA	60
P519_bkp	GAGGGAGACCTATAACATGAGC	TACTGTAAGGGAGATGTGTTGG	62
UP10K	TACAGACCATCACCGTTGCC	TTCACTCACCACCCATCCTC	65
UP3K	TTTCTCCTGACCGACCATCC	CACTGTCTTCTTAAAACCCACC	63
D1 promoter	CCTTGGGCATTTGCAACGAC	CGCATTTCCAAGAACGCCAC	64
D1 intron 1	TTCTTTGCTACTCACCCCC	CCCTTCCTCCTTCAGAAAATAC	61
D1 intron 4	TGGCTCAGAAACACCATCG	GGACATTCCACCCACCAAAG	65
Down 60 K	ACAATATCCCACCATCTCCTC	AACCCTAACACTCCCCATC	61
Eμ	CAGCCCTTGTTAATGGACTT	GGAAAGTTAAATGGGAGTGACC	65
3′ Cα HS4	TCCAGTCTGAAAAACAAGACC	ACCTCCCCCAATGCAAATC	63
3′ Cα HS3	AGGTCTCGACTTAGCACTG	GGCATGTTTCTCAGAACAGC	66