Table 1.

Stability of tetramer binding to CD8 T-cell receptors at 37°C


CD8 T cells/tetramers (medium)

Incubation time
1 h, %
2 h, %
3 h, %
8 h, %
24 h, %
LMP1/LMP1 (PBS)   92   94   95   86   86  
Flu58/LMP1 (PBS)   0.9   1.2   1.1   1.0   1.0  
LLO91/LLO91 (PBS)   68   78   82   —   69  
LMP1/LMP1 (serum)   98   94   86.4   71   28.8  
LLO91/LLO91 (serum)
 
88
 
66
 
62
 

 
10.6
 

CD8 T cells/tetramers (medium)

Incubation time
1 h, %
2 h, %
3 h, %
8 h, %
24 h, %
LMP1/LMP1 (PBS)   92   94   95   86   86  
Flu58/LMP1 (PBS)   0.9   1.2   1.1   1.0   1.0  
LLO91/LLO91 (PBS)   68   78   82   —   69  
LMP1/LMP1 (serum)   98   94   86.4   71   28.8  
LLO91/LLO91 (serum)
 
88
 
66
 
62
 

 
10.6
 

— indicates not determined.

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