Relative percentages of granzyme A- and B-expressing cells in human blood subsets
Cell type . | GzmA . | GzmB . |
|---|---|---|
| B cells | — | — |
| PMNs* | — | — |
| CD8+ cells | ||
| Circulating (CD8+CD56-) | ++ | ++ |
| Circulating (CD8+CD56+) | +++ | ++ |
| ConA/IL-2 | + | +++ |
| IL-2 | + | + |
| NK cells | ||
| Circulating (CD56+) | +++ | +++ |
| Circulating (CD56+8-) | +++ | ++/+++ |
| PBMCs (NK and CD8+) | ||
| Circulating | + | + |
| ConA/IL-2 | -/+ | +++ |
| CD4+ cells | ||
| Circulating | -/+ | -/+ |
| ConA/IL-2 | -/+ | +++ |
| IL-2 | -/+ | -/+ |
| CD3/CD28 | -/+ | ++/+++ |
| CD3/CD46 (Tr1) | -/+ | +++ |
| CD4+CD45RA+ (naive) | ||
| IL-2 | -/+ | -/+ |
| CD3/CD28 | -/+ | -/+ |
| CD3/CD46 | -/+ | +++ |
| CD4+CD45RO+ (memory) | ||
| IL-2 | -/+ | -/+ |
| CD3/CD28 | -/+ | +++ |
| CD3/CD46 | -/+ | +++ |
Cell type . | GzmA . | GzmB . |
|---|---|---|
| B cells | — | — |
| PMNs* | — | — |
| CD8+ cells | ||
| Circulating (CD8+CD56-) | ++ | ++ |
| Circulating (CD8+CD56+) | +++ | ++ |
| ConA/IL-2 | + | +++ |
| IL-2 | + | + |
| NK cells | ||
| Circulating (CD56+) | +++ | +++ |
| Circulating (CD56+8-) | +++ | ++/+++ |
| PBMCs (NK and CD8+) | ||
| Circulating | + | + |
| ConA/IL-2 | -/+ | +++ |
| CD4+ cells | ||
| Circulating | -/+ | -/+ |
| ConA/IL-2 | -/+ | +++ |
| IL-2 | -/+ | -/+ |
| CD3/CD28 | -/+ | ++/+++ |
| CD3/CD46 (Tr1) | -/+ | +++ |
| CD4+CD45RA+ (naive) | ||
| IL-2 | -/+ | -/+ |
| CD3/CD28 | -/+ | -/+ |
| CD3/CD46 | -/+ | +++ |
| CD4+CD45RO+ (memory) | ||
| IL-2 | -/+ | -/+ |
| CD3/CD28 | -/+ | +++ |
| CD3/CD46 | -/+ | +++ |
+/++/+++ indicates the relative percentage of cells demonstrating intracellular granzyme (Gzm) expression based on intracellular flow cytometry analysis. PMNs indicates polymorphonuclear cells; —, none detected; ++, 25% to 50%; +++, more than 75%; +, 10% to 25%; ++/+++, 50% to 75%; and -/+, less than 10%.
Grossman and Ley27