Single-marker analysis
Locus and genotype . | Low grade, range, % . | High grade, range, % . | All, range, % . | Controls, range, % . | E I, range, % . | E II-IV, range, % . |
|---|---|---|---|---|---|---|
| IL-1β -31 | ||||||
| C/C | 8-13.1 | 10-12.1 | 18-12.5 | 45-13.1 | 11-10.2 | 7-20.6 |
| C/T | 30-49.2 | 35-42.2 | 65-45.1 | 146-42.4 | 52-48.2 | 13-38.2 |
| T/T | 23-37.7 | 38-45.8 | 61-42.4 | 153-44.8 | 45-41.7 | 14-41.2 |
| IL-1β + 3954 | ||||||
| C/C | 4-6.7 | 3-3.7 | 7-5.0 | 24-7.2 | 3-2.8 | 3-9.4 |
| C/T | 20-33.3 | 26-32.1 | 46-32.6 | 121-36.3 | 37-34.6 | 9-28.1 |
| T/T | 36-60.0 | 52-64.2 | 88-62.4 | 188-56.5 | 67-62.6 | 20-62.5 |
| IL-1RN 86VNTR | ||||||
| 1/1 | 30-46.9 | 46-52.9 | 76-50.3 | 185-53.8 | 58-51.3 | 17-47.2 |
| 1/2 | 27-42.2 | 31-35.6 | 58-38.4 | 118-34.3 | 43-38.1 | 14-38.9 |
| 1/3 | 2-3.1 | 4-4.6 | 6-4.0 | 11-3.2 | 5-4.4 | 1-2.8 |
| 2/2 | 4-6.3 | 5-5.8 | 9-6.0 | 27-7.9 | 6-5.3 | 3-8.3 |
| 2/3 | 1-1.6 | 1-1.1 | 2-1.3 | 2-0.6 | 1-0.9 | 1-2.8 |
Locus and genotype . | Low grade, range, % . | High grade, range, % . | All, range, % . | Controls, range, % . | E I, range, % . | E II-IV, range, % . |
|---|---|---|---|---|---|---|
| IL-1β -31 | ||||||
| C/C | 8-13.1 | 10-12.1 | 18-12.5 | 45-13.1 | 11-10.2 | 7-20.6 |
| C/T | 30-49.2 | 35-42.2 | 65-45.1 | 146-42.4 | 52-48.2 | 13-38.2 |
| T/T | 23-37.7 | 38-45.8 | 61-42.4 | 153-44.8 | 45-41.7 | 14-41.2 |
| IL-1β + 3954 | ||||||
| C/C | 4-6.7 | 3-3.7 | 7-5.0 | 24-7.2 | 3-2.8 | 3-9.4 |
| C/T | 20-33.3 | 26-32.1 | 46-32.6 | 121-36.3 | 37-34.6 | 9-28.1 |
| T/T | 36-60.0 | 52-64.2 | 88-62.4 | 188-56.5 | 67-62.6 | 20-62.5 |
| IL-1RN 86VNTR | ||||||
| 1/1 | 30-46.9 | 46-52.9 | 76-50.3 | 185-53.8 | 58-51.3 | 17-47.2 |
| 1/2 | 27-42.2 | 31-35.6 | 58-38.4 | 118-34.3 | 43-38.1 | 14-38.9 |
| 1/3 | 2-3.1 | 4-4.6 | 6-4.0 | 11-3.2 | 5-4.4 | 1-2.8 |
| 2/2 | 4-6.3 | 5-5.8 | 9-6.0 | 27-7.9 | 6-5.3 | 3-8.3 |
| 2/3 | 1-1.6 | 1-1.1 | 2-1.3 | 2-0.6 | 1-0.9 | 1-2.8 |
Single-marker analysis of the proinflammatory haplotype IL-1β -31/IL-1 RN 86 VNTR. SNPs at IL-1β -31 and + 3954 were genotyped by allelic discrimination (TaqMan technology, ABI 7700, Aplera, Foster City, CA). IL-1RN 86 VNTR was genotyped by Southern blot after amplification. Alleles were sized relative to a 100-bp ladder (allele 1 = 4 repeats, allele 2 = 2 repeats, allele 3 = 5 repeats, allele 4 = 3 repeats, allele 5 = 6 repeats). Haplotype case-control analysis was performed using HAPMAX.9 Hardy-Weinberg equilibrium was confirmed for all polymorphisms tested in gastric lymphoma group and controls. Three types of analysis were performed: (1) all patients with primary gastric B-cell lymphoma were compared against controls, (2) patients separated in low-grade and high-grade lymphoma were compared against controls, and (3) patients with disease stage E I were compared against patients with disease stages E II to E IV. Statistical analysis was performed using SISA Binominal program (Uitenbroek, Daan G, Binomial, SISA, http://home.clara.net/sisa/binomial.htm).