Flow cytometric measurements of megakaryocyte cell diameter, granularity, and the expression levels of membrane glycoproteins
Ploidy class . | Cytometric measurement . | . | Relative expression of glycoproteins, platelet equivalent* . | . | . | |||
|---|---|---|---|---|---|---|---|---|
| . | Diameter, μm . | Granularity, mean channel . | GPIIb/IIIa . | GPIb . | GPIIIa . | |||
| 2N | 21.10 ± 3.8 | 17.6 ± 5.2 | 121.00 ± 47.4 | 65.70 ± 9.7 | 84.70 ± 25.8 | |||
| 4N | 28.30 ± 4.5 | 36.7 ± 9.4 | 157.30 ± 43.5 | 76.00 ± 14.8 | 103.00 ± 18.0 | |||
| 8N | 33.20 ± 4.3 | 55.8 ± 8.4 | 239.00 ± 42.6 | 99.7 ± 28.6 | 167.7 ± 21.1 | |||
| 16N | 37.80 ± 30.2 | 90.6 ± 7.1 | 381.70 ± 49.1 | 135.70 ± 30.7 | 320.00 ± 28.0 | |||
| 32N | 45.50 ± 4.2 | 142.0 ± 21.7 | 597.30 ± 127.3 | 211.70 ± 28.9 | 499.70 ± 75.2 | |||
| 64N | 55.80 ± 8.1 | 191.0 ± 32.1 | 893.50 ± 188.5 | 316.00 ± 14.7 | 711.50 ± 121.5 | |||
Ploidy class . | Cytometric measurement . | . | Relative expression of glycoproteins, platelet equivalent* . | . | . | |||
|---|---|---|---|---|---|---|---|---|
| . | Diameter, μm . | Granularity, mean channel . | GPIIb/IIIa . | GPIb . | GPIIIa . | |||
| 2N | 21.10 ± 3.8 | 17.6 ± 5.2 | 121.00 ± 47.4 | 65.70 ± 9.7 | 84.70 ± 25.8 | |||
| 4N | 28.30 ± 4.5 | 36.7 ± 9.4 | 157.30 ± 43.5 | 76.00 ± 14.8 | 103.00 ± 18.0 | |||
| 8N | 33.20 ± 4.3 | 55.8 ± 8.4 | 239.00 ± 42.6 | 99.7 ± 28.6 | 167.7 ± 21.1 | |||
| 16N | 37.80 ± 30.2 | 90.6 ± 7.1 | 381.70 ± 49.1 | 135.70 ± 30.7 | 320.00 ± 28.0 | |||
| 32N | 45.50 ± 4.2 | 142.0 ± 21.7 | 597.30 ± 127.3 | 211.70 ± 28.9 | 499.70 ± 75.2 | |||
| 64N | 55.80 ± 8.1 | 191.0 ± 32.1 | 893.50 ± 188.5 | 316.00 ± 14.7 | 711.50 ± 121.5 | |||
Data are shown as mean and standard deviation (I SD).
The fluorescence level of marrow MKs relative to that of circulating platelets.