Contribution of Notch1-deficient and wild-type cells to definitive hematopoiesis in chimeric mice
Chimera age . | Organ assayed . | Notch1 genotype . | No. of chimeras analyzed . | No. of different ES lines . | Percent LacZ+ CFU . | Range . |
|---|---|---|---|---|---|---|
| 9.5 to 10.5 | YS | +/+ | 1 | 1 | 29 | |
| -/- | 6 | 3 | 35 ± 19 | 15-66 | ||
| 11.5 to 12.5 | YS | +/+ | 1 | 1 | 33 | |
| -/- | 8 | 2 | 4 ± 5 | 0-14 | ||
| 13.5 to 14.5 | FL | +/+ | 1 | 1 | 51 | |
| -/- | 2 | 1 | 4.5 | 4-5 | ||
| 15.5 to postnatal | FL/BM | +/+ | 5 | 2 | 40 ± 17 | 25-68 |
| -/- | 10 | 3 | 0 | 0 |
Chimera age . | Organ assayed . | Notch1 genotype . | No. of chimeras analyzed . | No. of different ES lines . | Percent LacZ+ CFU . | Range . |
|---|---|---|---|---|---|---|
| 9.5 to 10.5 | YS | +/+ | 1 | 1 | 29 | |
| -/- | 6 | 3 | 35 ± 19 | 15-66 | ||
| 11.5 to 12.5 | YS | +/+ | 1 | 1 | 33 | |
| -/- | 8 | 2 | 4 ± 5 | 0-14 | ||
| 13.5 to 14.5 | FL | +/+ | 1 | 1 | 51 | |
| -/- | 2 | 1 | 4.5 | 4-5 | ||
| 15.5 to postnatal | FL/BM | +/+ | 5 | 2 | 40 ± 17 | 25-68 |
| -/- | 10 | 3 | 0 | 0 |
Contribution of Notch1-deficient (Notch1Δ1 allele) ES cells (-/-) and control wild-type (+/+) ES cells to definitive hematopoietic CFU progenitors in yolk sac (YS), fetal liver (FL), and bone marrow (BM) of high-percentage chimeras at various stages of development. Colonies were stained with X-gal stain to determine the percentages of overall colonies obtained from ES-derived (LacZ+) and embryo-derived (LacZ-) progenitors. We did not observe any bias in the distribution of erythroid, myeloid (macrophage or monocyte/granulocyte), or mixed colonies in the LacZ+ and LacZ- populations (not shown). For each age range, the number of chimera analyzed and the number of different ES lines used to generate these chimeras are indicated, as well as the average, standard deviation, and range of percent contribution of LacZ+ CFUs.