Frequency of electron light MK and of neutrophil emperipolesis in the marrow and spleen from normal and GATA-1low mice
. | Bone marrow . | . | Spleen . | . | ||
|---|---|---|---|---|---|---|
| Mouse/age, mo . | Light MK, % . | MK embedded with neutrophils, % . | Light MK, % . | MK embedded with neutrophils, % . | ||
| Wild-type | ||||||
| 4-6 | 100 | < 1 | 100 | 2.3 ± 0.2 | ||
| 8-9 | 100 | < 1 | 100 | < 1 | ||
| GATA-1low | ||||||
| 4-6 (disease-free) | 60 ± 5 | 34 ± 4 | 45 ± 4 | 16 ± 1 | ||
| 8-9 (early disease) | 53 ± 4 | 32 ± 2 | 63 ± 7 | 16 ± 2 | ||
| 12-15 (myelofibrotic) | NA | NA | 34 ± 3 | 19 ± 6 | ||
. | Bone marrow . | . | Spleen . | . | ||
|---|---|---|---|---|---|---|
| Mouse/age, mo . | Light MK, % . | MK embedded with neutrophils, % . | Light MK, % . | MK embedded with neutrophils, % . | ||
| Wild-type | ||||||
| 4-6 | 100 | < 1 | 100 | 2.3 ± 0.2 | ||
| 8-9 | 100 | < 1 | 100 | < 1 | ||
| GATA-1low | ||||||
| 4-6 (disease-free) | 60 ± 5 | 34 ± 4 | 45 ± 4 | 16 ± 1 | ||
| 8-9 (early disease) | 53 ± 4 | 32 ± 2 | 63 ± 7 | 16 ± 2 | ||
| 12-15 (myelofibrotic) | NA | NA | 34 ± 3 | 19 ± 6 | ||
The mice are divided into age groups to take into account the progression of myelofibrosis in the GATA-1low mutants. The results are obtained by analyzing at least 50 individual MKs per experimental point and are presented as mean ± SD of data obtained in 3 individual mice per age group.
NA indicates electron microscopy not available because of fibrosis.