Differences in the incidence of tumor relapses following GCV treatment in vivo
Transplanted cells . | No. of mice with complete remission, +GCV . | No. of mice with tumor relapse after GCV treatment . | Mean survival time after tumor relapse, d . | Escape mechanism of independent tumors . |
|---|---|---|---|---|
| EL-4, mock | 1/5 | 5/5 | 23.0 ± 7.2 | NA |
| Mass culture, MO3N | 0/5 | 5/5 | 19.0 ± 2.4 | NA |
| Mass culture, MO3TIN | 2/5 | 5/5 | 26.6 ± 6.7 | ND |
| Clone no. 1, MO3TIN; Exp 1 | 5/5 | 2/5 | 35.5 ± 1.5 | 2 CD/CL |
| Clone no. 1, MO3TIN; Exp 2 | 5/5 | 0/5 | NA | NA |
| Clone no. 2, MO3TIN | 4/5 | 5/5 | 31.8 ± 8.2 | 3 CD/CL, 2 PR |
| Clone no. 3, MO3TIN; Exp 1 | 5/5 | 3/5 | 28.3 ± 1.8 | 2 CD/CL, 1 PR |
| Clone no. 3, MO3TIN; Exp 2 | 5/5 | 3/5 | 34.3 ± 6.4 | ND |
| Clone no. 4, MO3TIN; Exp 1 | 5/5 | 4/5 | 24.8 ± 1.1 | 4 GS |
| Clone no. 4, MO3TIN; Exp 2 | 5/5 | 5/5 | 27.2 ± 3.4 | 5 GS |
| Clone no. 5, MO3TIN; Exp 1 | 5/5 | 1/5 | 37.0 | 1 PR |
| Clone no. 5, MO3TIN; Exp 2 | 5/5 | 0/5 | NA | NA |
| Clone no. 6, MO3TIN | 5/5 | 2/5 | 32.5 ± 1.5 | 2 CD/CL |
Transplanted cells . | No. of mice with complete remission, +GCV . | No. of mice with tumor relapse after GCV treatment . | Mean survival time after tumor relapse, d . | Escape mechanism of independent tumors . |
|---|---|---|---|---|
| EL-4, mock | 1/5 | 5/5 | 23.0 ± 7.2 | NA |
| Mass culture, MO3N | 0/5 | 5/5 | 19.0 ± 2.4 | NA |
| Mass culture, MO3TIN | 2/5 | 5/5 | 26.6 ± 6.7 | ND |
| Clone no. 1, MO3TIN; Exp 1 | 5/5 | 2/5 | 35.5 ± 1.5 | 2 CD/CL |
| Clone no. 1, MO3TIN; Exp 2 | 5/5 | 0/5 | NA | NA |
| Clone no. 2, MO3TIN | 4/5 | 5/5 | 31.8 ± 8.2 | 3 CD/CL, 2 PR |
| Clone no. 3, MO3TIN; Exp 1 | 5/5 | 3/5 | 28.3 ± 1.8 | 2 CD/CL, 1 PR |
| Clone no. 3, MO3TIN; Exp 2 | 5/5 | 3/5 | 34.3 ± 6.4 | ND |
| Clone no. 4, MO3TIN; Exp 1 | 5/5 | 4/5 | 24.8 ± 1.1 | 4 GS |
| Clone no. 4, MO3TIN; Exp 2 | 5/5 | 5/5 | 27.2 ± 3.4 | 5 GS |
| Clone no. 5, MO3TIN; Exp 1 | 5/5 | 1/5 | 37.0 | 1 PR |
| Clone no. 5, MO3TIN; Exp 2 | 5/5 | 0/5 | NA | NA |
| Clone no. 6, MO3TIN | 5/5 | 2/5 | 32.5 ± 1.5 | 2 CD/CL |
± indicates SEM; NA, not applicable; ND, not determined; CD/CL, chromosomal deletion and/or chromosomal loss; PR, postinsertional recombination; and GS, gene silencing.