Inhibition of [3H]ADP transport into platelet dense vesicles
Compound . | Concentration, μM . | [3H]ADP transport, % of control . |
|---|---|---|
| None (control) | NA | 100 |
| Dipyridamole | 10 | 52.7 ± 5.2 |
| 100 | 8.8 ± 0.4 | |
| MK571 | 100 | 40.7 ± 3.9 |
| cGMP | 100 | 28.7 ± 2.3 |
Compound . | Concentration, μM . | [3H]ADP transport, % of control . |
|---|---|---|
| None (control) | NA | 100 |
| Dipyridamole | 10 | 52.7 ± 5.2 |
| 100 | 8.8 ± 0.4 | |
| MK571 | 100 | 40.7 ± 3.9 |
| cGMP | 100 | 28.7 ± 2.3 |
Platelet-dense membrane vesicles (100 μg protein) were incubated with [3H]ADP (1 μM) in the presence of 0.4 mM ATP or 0.4 mM ATP + 1 mM orthovanadate for 2 minutes, and the difference in the vesicle-associated radioactivity was calculated. The compounds listed were added in the given concentrations to incubations with and without orthovanadate. The difference is given as percent of control (mean values ± SD from 3 determinations). The control vanadate-sensitive [3H]ADP transport at 2 minutes in these experiments was 3.0 ± 0.4 pmol/mg protein-1.