RQ-PCR primer and probe sequences
Transcript . | Sense . | Antisense . | Probe (FAM-TAMRA) . |
|---|---|---|---|
| ABL | TGGAGATAACACTCTAAGCATAACTAAAGGT | GATGTAGTTGCTTGGGACCCA | CCATTTTTGGTTTGGGCTTCACACCATT |
| RAG-1 | AGCCTGCTGAGCAAGGTACC | GAACTGAGTCCCAAGGTGGG | AGCCAGCATGGCAGCCTCTTTCC |
| pTα | TTGGGTGTCCAGCCCTACC | GCCATAGGTGAAGGCATCCA | CAGCCGGCAATGGCAGTGCA |
| HOX11 | CGCAGATACACAAAGGACAGGTT | CGTGCGCGGCTTCTTCT | AGAACCGGACGCCCCCCAAG |
| TAL1 | CTCTACAGCCTCAGCCAGCC | CAAAGGCCCCGTTCACATT | CGGTAGCCTTCCCTATGTTCACCACC |
| LYL1 | TCACCCCTTCCTCAACAGTGT | CGGGCCACCTTCTGGG | ACATTGGGCCAGCAGGACCTTTTAGC |
| LMO2 | GCCATCGAAAGGAAGAGCCT | AAGTAGCGGTCCCCGATGTT | CCTGCTGACATGCGGCT |
| LMO1 | CCAAGGCCAACCTCATCCT | AGTTCCCTGTGGTGCCAAAG | TGCCGACGCGACTACCTGAGGC |
Transcript . | Sense . | Antisense . | Probe (FAM-TAMRA) . |
|---|---|---|---|
| ABL | TGGAGATAACACTCTAAGCATAACTAAAGGT | GATGTAGTTGCTTGGGACCCA | CCATTTTTGGTTTGGGCTTCACACCATT |
| RAG-1 | AGCCTGCTGAGCAAGGTACC | GAACTGAGTCCCAAGGTGGG | AGCCAGCATGGCAGCCTCTTTCC |
| pTα | TTGGGTGTCCAGCCCTACC | GCCATAGGTGAAGGCATCCA | CAGCCGGCAATGGCAGTGCA |
| HOX11 | CGCAGATACACAAAGGACAGGTT | CGTGCGCGGCTTCTTCT | AGAACCGGACGCCCCCCAAG |
| TAL1 | CTCTACAGCCTCAGCCAGCC | CAAAGGCCCCGTTCACATT | CGGTAGCCTTCCCTATGTTCACCACC |
| LYL1 | TCACCCCTTCCTCAACAGTGT | CGGGCCACCTTCTGGG | ACATTGGGCCAGCAGGACCTTTTAGC |
| LMO2 | GCCATCGAAAGGAAGAGCCT | AAGTAGCGGTCCCCGATGTT | CCTGCTGACATGCGGCT |
| LMO1 | CCAAGGCCAACCTCATCCT | AGTTCCCTGTGGTGCCAAAG | TGCCGACGCGACTACCTGAGGC |
HOX11L2 primers and probes are as described.30 SIL-TAL1 detection was performed by RT-PCR as described.56