Primitive erythroid colonies develop from Scl+ cells
.
Primitive erythroid colonies
.
Nonfractionated
425 ± 30
hCD4-VE-cadherin-
3 ± 2
hCD4+VE-cadherin+
589 ± 61
hCD4+VE-cadherin-
1636 ± 48
.
Primitive erythroid colonies
.
Nonfractionated
425 ± 30
hCD4-VE-cadherin-
3 ± 2
hCD4+VE-cadherin+
589 ± 61
hCD4+VE-cadherin-
1636 ± 48
Scl+/hCD4 ES cells were differentiated on OP9 for 4 days; sorted for hCD4-VE-cadherin-, hCD4+VE-cadherin+, or hCD4+VE-cadherin-; and replated for primitive erythroid colonies (6 × 104 cells/mL). The resulting primitive erythroid colonies were counted 4 days later. Data are expressed as mean values ± SD.