VE-cadherin is not a marker for definitive hematopoietic cells
. | E . | M . | E + M . |
---|---|---|---|
Nonfractionated | 1942 ± 82 | 206 ± 20 | 216 ± 40 |
VE-cadherin- | 2010 ± 93 | 190 ± 59 | 280 ± 45 |
VE-cadherin+ | 134 ± 20 | 468 ± 34 | 100 ± 6 |
. | E . | M . | E + M . |
---|---|---|---|
Nonfractionated | 1942 ± 82 | 206 ± 20 | 216 ± 40 |
VE-cadherin- | 2010 ± 93 | 190 ± 59 | 280 ± 45 |
VE-cadherin+ | 134 ± 20 | 468 ± 34 | 100 ± 6 |
Scl+/hCD4 ES cells were differentiated on OP9 for 6 days and FACS sorted for VE-cadherin+ and VE-cadherin- cells and subjected to hematopoietic replating (6 × 104 cells/mL). The resulting hematopoietic colonies were counted 6 days later. Data are expressed as mean values ± SD. Three independent sortings show similar results.
E indicates erythroid; M, macrophage; and E + M, erythroid/macrophage mixed colonies.