Table 2.

VE-cadherin is not a marker for definitive hematopoietic cells




E

M

E + M
Nonfractionated   1942 ± 82   206 ± 20   216 ± 40  
VE-cadherin-  2010 ± 93   190 ± 59   280 ± 45  
VE-cadherin+
 
134 ± 20
 
468 ± 34
 
100 ± 6
 



E

M

E + M
Nonfractionated   1942 ± 82   206 ± 20   216 ± 40  
VE-cadherin-  2010 ± 93   190 ± 59   280 ± 45  
VE-cadherin+
 
134 ± 20
 
468 ± 34
 
100 ± 6
 

Scl+/hCD4 ES cells were differentiated on OP9 for 6 days and FACS sorted for VE-cadherin+ and VE-cadherin- cells and subjected to hematopoietic replating (6 × 104 cells/mL). The resulting hematopoietic colonies were counted 6 days later. Data are expressed as mean values ± SD. Three independent sortings show similar results.

E indicates erythroid; M, macrophage; and E + M, erythroid/macrophage mixed colonies.

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