Primers
Genes . | Primer sequences . | Product size, bp . |
|---|---|---|
| ISH probe primers | ||
| Ephrin B2 | 5′-TCC GTG TGG AGT ACT GCT G-3′ | 296 |
| 5′-TCT GGT TTG GCA CAG TTG AG-3′ | ||
| EphB4 | 5′-CTT TGG AAG AGA CCC TGC TG-3′ | 297 |
| 5′-AGA CGG TGA AGG TCT CCT TG-3′ | ||
| RT-PCR primers | ||
| Ephrin B2 | 5′-AGA CAA GAG CCA TGA AGA TC-3′ | 200 |
| 5′-GGA TCC CAC TTC GGA CCC GAG-3′ | ||
| EphB4 | 5′-TCA GGT CAC TGC ATT GAA CGG G-3′ | 400 |
| 5′-AAC TCG CTC TCA TCC AGT T-3′ | ||
| vGPCR | 5′-ATG GCG GCC GAG GAT TTC CTA ACC-3′ | 434 |
| 5′-AGG TAC CTC ACT AGA CTG ACG CAC-3′ | ||
| LANA1 | 5′-TCC CCC TAG ATG TGA CTT CG-3′ | 406 |
| 5′-ATC CTC CTC GTC ATC CTC CT-3′ | ||
| GAPDH | 5′-TGA AGG TCG GAG TCA ACG GAT TTG GT-3′ | 983 |
| 5′-CAT GTG GGC CAT GAG GTC CAC CAC-3′ | ||
| β-actin | 5′-GTG GGG CGC CCC AGG CAC CA-3′ | 546 |
| 5′-CTC CTT AAT GTC ACG CAC GAT TTC-3′ |
Genes . | Primer sequences . | Product size, bp . |
|---|---|---|
| ISH probe primers | ||
| Ephrin B2 | 5′-TCC GTG TGG AGT ACT GCT G-3′ | 296 |
| 5′-TCT GGT TTG GCA CAG TTG AG-3′ | ||
| EphB4 | 5′-CTT TGG AAG AGA CCC TGC TG-3′ | 297 |
| 5′-AGA CGG TGA AGG TCT CCT TG-3′ | ||
| RT-PCR primers | ||
| Ephrin B2 | 5′-AGA CAA GAG CCA TGA AGA TC-3′ | 200 |
| 5′-GGA TCC CAC TTC GGA CCC GAG-3′ | ||
| EphB4 | 5′-TCA GGT CAC TGC ATT GAA CGG G-3′ | 400 |
| 5′-AAC TCG CTC TCA TCC AGT T-3′ | ||
| vGPCR | 5′-ATG GCG GCC GAG GAT TTC CTA ACC-3′ | 434 |
| 5′-AGG TAC CTC ACT AGA CTG ACG CAC-3′ | ||
| LANA1 | 5′-TCC CCC TAG ATG TGA CTT CG-3′ | 406 |
| 5′-ATC CTC CTC GTC ATC CTC CT-3′ | ||
| GAPDH | 5′-TGA AGG TCG GAG TCA ACG GAT TTG GT-3′ | 983 |
| 5′-CAT GTG GGC CAT GAG GTC CAC CAC-3′ | ||
| β-actin | 5′-GTG GGG CGC CCC AGG CAC CA-3′ | 546 |
| 5′-CTC CTT AAT GTC ACG CAC GAT TTC-3′ |