MGd effect on normal PBLs
Cells . | % cells staining PI+ following incubation with MGd 50 μM + AA 50 μM, mean ± SD* . | % cells staining PI+ following incubation with MGd 50 μM + AA 100 μM, mean ± SD* . |
|---|---|---|
| PBLs†‡ | 36.5 ± 15.0 | 65.3 ± 12.5 |
| PBLs + CAT† | ND | 10.6 ± 1.1 |
| 8226-RPMI§ | 64.9 ± 0.4 | 98.2 ± 0.2 |
| DOX-10V§ | 13.8 ± 1.1 | 89.3 ± 1.1 |
| C2E3§ | 97.0 ± 0.1 | ND |
| 1-130§ | 98.7 ± 0.2 | 99.3 ± 0.4 |
Cells . | % cells staining PI+ following incubation with MGd 50 μM + AA 50 μM, mean ± SD* . | % cells staining PI+ following incubation with MGd 50 μM + AA 100 μM, mean ± SD* . |
|---|---|---|
| PBLs†‡ | 36.5 ± 15.0 | 65.3 ± 12.5 |
| PBLs + CAT† | ND | 10.6 ± 1.1 |
| 8226-RPMI§ | 64.9 ± 0.4 | 98.2 ± 0.2 |
| DOX-10V§ | 13.8 ± 1.1 | 89.3 ± 1.1 |
| C2E3§ | 97.0 ± 0.1 | ND |
| 1-130§ | 98.7 ± 0.2 | 99.3 ± 0.4 |
AA indicates ascorbic acid; CAT, catalase; ND, not done.
Percent cells staining PI+ following 20 or 24 hours of incubation
Results shown (means and SD) were averaged from 3 individual healthy donors with each experiment done in triplicate
Ascorbate/mannitol, MGd/mannitol, and catalase/mannitol controls resulted in 10% to 14% PI+ cells following 24-hour incubation with PBLs
Results shown (means and SD) were averaged from 3 or more independent experiments done in triplicate for each time point