PML and TRAIL induction following IFN-α treatment of MM lines
MM cell line . | Growth inhibition response . | Induction of PML . | TRAIL protein expression . |
|---|---|---|---|
| OPM1 | No | No | No |
| OPM2 | No | No | No |
| XG1 | No | No | No |
| Delta47 | No | No | No |
| ANBL6 | Yes | Yes | Yes |
| MM144 | Yes | Yes | Yes |
| H929 | Yes | Yes | Yes |
| 8226 | Yes | Yes | Yes |
| XG2 | Yes | Yes | Yes |
MM cell line . | Growth inhibition response . | Induction of PML . | TRAIL protein expression . |
|---|---|---|---|
| OPM1 | No | No | No |
| OPM2 | No | No | No |
| XG1 | No | No | No |
| Delta47 | No | No | No |
| ANBL6 | Yes | Yes | Yes |
| MM144 | Yes | Yes | Yes |
| H929 | Yes | Yes | Yes |
| 8226 | Yes | Yes | Yes |
| XG2 | Yes | Yes | Yes |
MM cell lines were treated with or without 2000 U/mL IFN-α for 48 hours. Growth inhibition was measured by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulphenyl)-2H-tetrazolium (MTS) assay as described in “Materials and methods.” PML induction was assessed by Western blot and confocal image analysis. TRAIL was detected by Western blotting.