Effects of ion channel and electron transport complex inhibitors on p21-IRS–induced apoptosis
. | Inhibitor (dose) . | Percent inhibition of apoptosis ± SD . |
|---|---|---|
| Na+ channel | QX222 (15 mM) | 37.0 ± 2.2 |
| Amiloride (1.5 mM) | 46.0 ± 1.4 | |
| Ca2+ channel | Econazole (20 μM) | 44.0 ± 2.3 |
| Nicardipine (20 μM) | 71.0 ± 6.2 | |
| Ni2+ (0.5 mM) | 30.0 ± 1.1 | |
| EDTA (10 mM) | 68.0 ± 0.5 | |
| EGTA (5 mM) | 55.0 ± 6.6 | |
| K+ channel | TEA (50 mM) | 73.6 ± 1.1 |
| Apamin (50 μM) | 84.0 ± 8.2 | |
| High K+ buffer | 83.0 ± 3.8 | |
| Electron transport complex | ||
| Complex I | Rotenone (20 μM) | 49.0 ± 1.6 |
| Complex II | TTFA (500 μM) | 52.0 ± 3.6 |
| Complex III | Actimycin A (30 μM) | 6.5 ± 5.1 |
| Myxothiazol (10 μM) | 0 | |
| Stigmatellin (10 μM) | 0 | |
| Complex IV | KCN (7.5 mM) | 53.0 ± 3.5 |
. | Inhibitor (dose) . | Percent inhibition of apoptosis ± SD . |
|---|---|---|
| Na+ channel | QX222 (15 mM) | 37.0 ± 2.2 |
| Amiloride (1.5 mM) | 46.0 ± 1.4 | |
| Ca2+ channel | Econazole (20 μM) | 44.0 ± 2.3 |
| Nicardipine (20 μM) | 71.0 ± 6.2 | |
| Ni2+ (0.5 mM) | 30.0 ± 1.1 | |
| EDTA (10 mM) | 68.0 ± 0.5 | |
| EGTA (5 mM) | 55.0 ± 6.6 | |
| K+ channel | TEA (50 mM) | 73.6 ± 1.1 |
| Apamin (50 μM) | 84.0 ± 8.2 | |
| High K+ buffer | 83.0 ± 3.8 | |
| Electron transport complex | ||
| Complex I | Rotenone (20 μM) | 49.0 ± 1.6 |
| Complex II | TTFA (500 μM) | 52.0 ± 3.6 |
| Complex III | Actimycin A (30 μM) | 6.5 ± 5.1 |
| Myxothiazol (10 μM) | 0 | |
| Stigmatellin (10 μM) | 0 | |
| Complex IV | KCN (7.5 mM) | 53.0 ± 3.5 |