Peripheral blood hematology and BM CFU-Cs for HW80 background STAT5ab-/- mice
. | Wild-type . | STAT5ab-/- . |
|---|---|---|
| Peripheral blood hematology | ||
| White blood cells/μL | 11320 ± 2947 | 4084 ± 916* |
| Hematocrit, % | 45 ± 5 | 37 ± 0.6† |
| Absolute neutrophil count/μL | 1806 ± 1145 | 2067 ± 1178 |
| Absolute lymphocyte count/μL | 9142 ± 3307 | 1347 ± 823* |
| Absolute monocyte count/μL | 373 ± 194 | 1137 ± 315* |
| BM CFU-C/105 cells | ||
| GM-CSF | 140 ± 43 | 145 ± 61 |
| IL-3; IL-6, SCF, EPO | 199 ± 27 | 163 ± 82 |
. | Wild-type . | STAT5ab-/- . |
|---|---|---|
| Peripheral blood hematology | ||
| White blood cells/μL | 11320 ± 2947 | 4084 ± 916* |
| Hematocrit, % | 45 ± 5 | 37 ± 0.6† |
| Absolute neutrophil count/μL | 1806 ± 1145 | 2067 ± 1178 |
| Absolute lymphocyte count/μL | 9142 ± 3307 | 1347 ± 823* |
| Absolute monocyte count/μL | 373 ± 194 | 1137 ± 315* |
| BM CFU-C/105 cells | ||
| GM-CSF | 140 ± 43 | 145 ± 61 |
| IL-3; IL-6, SCF, EPO | 199 ± 27 | 163 ± 82 |
Four- to 8-week-old STAT5ab-/- (n = 4) and littermate wild-type (n = 4) mice were bled and analyzed for peripheral blood hematology. These mice were also humanely killed and the BM cells were harvested and plated in methylcellulose medium to assay for the CFU-C frequency in response to either GM-CSF or the cytokine cocktail of IL-3, IL-6, SCF, and EPO. The values for wild-type and STAT5ab-/- columns represent the absolute numbers of the description in the left-hand column.
P < .003 for comparison of wild-type versus STAT5ab-/-.
P = .02 for comparison of wild-type versus STAT5ab-/-.