Angiogenic effects of GAS6 and Axl-Ig on the chick CAM
Stimulation . | No. of CAM examined . | Vessel/mm3* . | Statistical significance† . |
|---|---|---|---|
| Vehicle | 10 | 4.58 ± 1.16 | ‡ |
| VEGF-A165§ | 10 | 14.46 ± 2.52 | ¶ |
| VEGF-A165 + GAS6 | 5 | 7.03 ± 0.47 | ‡¶ |
| FGF-2 | 5 | 16.32 ± 1.26 | ¶ |
| FGF-2 + GAS6 | 6 | 15.50 ± 2.12 | ¶ |
| VEGF-A165 + GAS6 + Axl-Ig | 3 | 12.14 ± 2.62 | ¶ |
| GAS6 | 4 | 4.00 ± 0.62 | ‡ |
| GAS6 + Axl-Ig | 4 | 4.21 ± 1.63 | ‡ |
| Axl-Ig | 4 | 4.14 ± 1.43 | ‡ |
Stimulation . | No. of CAM examined . | Vessel/mm3* . | Statistical significance† . |
|---|---|---|---|
| Vehicle | 10 | 4.58 ± 1.16 | ‡ |
| VEGF-A165§ | 10 | 14.46 ± 2.52 | ¶ |
| VEGF-A165 + GAS6 | 5 | 7.03 ± 0.47 | ‡¶ |
| FGF-2 | 5 | 16.32 ± 1.26 | ¶ |
| FGF-2 + GAS6 | 6 | 15.50 ± 2.12 | ¶ |
| VEGF-A165 + GAS6 + Axl-Ig | 3 | 12.14 ± 2.62 | ¶ |
| GAS6 | 4 | 4.00 ± 0.62 | ‡ |
| GAS6 + Axl-Ig | 4 | 4.21 ± 1.63 | ‡ |
| Axl-Ig | 4 | 4.14 ± 1.43 | ‡ |
Images photographed as described in “Materials and methods” were digitally recorded, and the number of vessels was quantified with an imaging software in 3 randomly selected areas (1 mm3).
Data were analyzed by ANOVA (F = 31.29) and Student-Newman-Keuls test.
P < .05 within indicated stimulations and CAM stimulated by VEGF-A165.
VEGF-A165 or FGF-2, GAS6, and Axl-Ig were used at 50 ng, 20 ng, and 200 ng per sponge, respectively.
P < .05 within indicated stimulations and CAM stimulated by vehicle alone.