Characteristics of macrophage progenitors from the BM of BCL-6−/− mice
. | No. colonies/1 × 104 BM cells . | . | |
|---|---|---|---|
| Cytokine addition . | BCL-6−/− . | BCL-6+/+ . | |
| Control medium | 8.4 ± 2.5† | 0 ± 0 | |
| + ctrl Ab (no. 1)* | 9.4 ± 2.3 | 0 ± 0 | |
| + anti-IL-6 (no. 1)* | 1.0 ± 0.4 | 0 ± 0 | |
| + ctrl Ab (no. 2)* | 9.0 ± 3.8 | 0 ± 0 | |
| + anti-IL-6 (no. 2)* | 0.8 ± 0.7 | 0 ± 0 | |
| mIL-6 | 21.0 ± 4.7† | 0 ± 0 | |
| CSF-1 medium | 42.3 ± 9.2† | 15.1 ± 4.2 | |
| CSF-1 + SCF medium | 41.4 ± 7.9 | 27.3 ± 7.2 | |
. | No. colonies/1 × 104 BM cells . | . | |
|---|---|---|---|
| Cytokine addition . | BCL-6−/− . | BCL-6+/+ . | |
| Control medium | 8.4 ± 2.5† | 0 ± 0 | |
| + ctrl Ab (no. 1)* | 9.4 ± 2.3 | 0 ± 0 | |
| + anti-IL-6 (no. 1)* | 1.0 ± 0.4 | 0 ± 0 | |
| + ctrl Ab (no. 2)* | 9.0 ± 3.8 | 0 ± 0 | |
| + anti-IL-6 (no. 2)* | 0.8 ± 0.7 | 0 ± 0 | |
| mIL-6 | 21.0 ± 4.7† | 0 ± 0 | |
| CSF-1 medium | 42.3 ± 9.2† | 15.1 ± 4.2 | |
| CSF-1 + SCF medium | 41.4 ± 7.9 | 27.3 ± 7.2 | |
Results are shown as the mean ± SD of colony numbers.
Ab indicates antibody.
For each assay, 2 to 3 pairs of litter-matched mice were used. Colony counts are averaged from 3 plates/mouse (n = 6-9).
The difference between BCL-6+/+ and BCL-6−/− cells is statistically significant (2-tailed Student t test, P < .05).