Inhibitory effect of IMD-0354 on cell cycle progression
Reagent, μM . | Proliferation index, % . |
|---|---|
| None | 43.0 ± 1.2 |
| IMD-0354, 0.5 | 21.6 ± 0.6* |
| IMD-0354, 1 | 18.3 ± 1.3* |
| ST1571, 0.5 | 44.2 ± 0.5 |
| ST1571, 1 | 42.7 ± 0.8 |
Reagent, μM . | Proliferation index, % . |
|---|---|
| None | 43.0 ± 1.2 |
| IMD-0354, 0.5 | 21.6 ± 0.6* |
| IMD-0354, 1 | 18.3 ± 1.3* |
| ST1571, 0.5 | 44.2 ± 0.5 |
| ST1571, 1 | 42.7 ± 0.8 |
Cells were incubated at the concentration of 106 cells/mL in α-MEM containing 10% FCS and antibiotics with or without indicated concentrations of IMD-0354 or ST1571. We analyzed DNA histograms in the G0/G1, S, and G2/M phases in HMC-1 cells stained with PI with the use of a flow cytometer and calculated the ratio of a 4N cell population in all cells with the use of the following formula: proliferation index = (no. cells in S, G2, and M phases)/no. cells in all phases × 100 (%).
P < .01 compared with None