Primers used for RT-PCR
Transcript . | Primer . | Sequence (5′ to 3′) . | Product, bp . |
|---|---|---|---|
| GAPDH | 5′ | TGCAGTGGCAAAGTGGAGATT | 791 |
| 3′ | TTGAAGTCGCAGGAGACAACCT | ||
| LYVE-1 | 5′ | ACCTCGTGCAAGACCTTTCCAT | 963 |
| 3′ | AGGAACTGACAGTGGCTTGCTT | ||
| Prox1 | 5′ | TGCCCTTGATGGCTTATCCA | 638 |
| 3′ | CGCGATGATGGCATTGAA | ||
| Podoplanin | 5′ | ATGAATCTACTGGCAAGGCACC | 204 |
| 3′ | GCGTTTCATCCCCTGCATTA | ||
| mVEGFR3 | 5′ | AGCGCATGTGCCCAGTATTGT | 266 |
| 3′ | GGACCCAGAAGAAAACTGCGA | ||
| hVEGFR3 | 5′ | CTGGTAGGGGAGAAGCTGGTC | 402 |
| 3′ | GGACAGTGCCTTTCCATCCTT | ||
| PECAM-1 | 5′ | AGCTAGCAAGAAGCAGGAAGGA | 307 |
| 3′ | GTAATGGCTGTTGGCTTCCACA | ||
| VE-cadherin | 5′ | CGCCAACATCACGGTCAAGTA | 405 |
| 3′ | CCGCGCACAGAATTAAGCA | ||
| αSMA | 5′ | CCGATAGAACACGGCATCATCA | 555 |
| 3′ | GCGTTCGTTTCCAATGGTGA |
Transcript . | Primer . | Sequence (5′ to 3′) . | Product, bp . |
|---|---|---|---|
| GAPDH | 5′ | TGCAGTGGCAAAGTGGAGATT | 791 |
| 3′ | TTGAAGTCGCAGGAGACAACCT | ||
| LYVE-1 | 5′ | ACCTCGTGCAAGACCTTTCCAT | 963 |
| 3′ | AGGAACTGACAGTGGCTTGCTT | ||
| Prox1 | 5′ | TGCCCTTGATGGCTTATCCA | 638 |
| 3′ | CGCGATGATGGCATTGAA | ||
| Podoplanin | 5′ | ATGAATCTACTGGCAAGGCACC | 204 |
| 3′ | GCGTTTCATCCCCTGCATTA | ||
| mVEGFR3 | 5′ | AGCGCATGTGCCCAGTATTGT | 266 |
| 3′ | GGACCCAGAAGAAAACTGCGA | ||
| hVEGFR3 | 5′ | CTGGTAGGGGAGAAGCTGGTC | 402 |
| 3′ | GGACAGTGCCTTTCCATCCTT | ||
| PECAM-1 | 5′ | AGCTAGCAAGAAGCAGGAAGGA | 307 |
| 3′ | GTAATGGCTGTTGGCTTCCACA | ||
| VE-cadherin | 5′ | CGCCAACATCACGGTCAAGTA | 405 |
| 3′ | CCGCGCACAGAATTAAGCA | ||
| αSMA | 5′ | CCGATAGAACACGGCATCATCA | 555 |
| 3′ | GCGTTCGTTTCCAATGGTGA |
GAPDH indicates glyceraldehyde-3-phosphate dehydrogenase.