Percentages of YFP+ bCMV/bLCV-specific CD4 T cells of total YFP+ CD4 T cells and, for comparison, percentages of YFP- bCMV/bLCV-specific CD4 T cells of total YFP- CD4 T cells
. | bCMV-specific CD4 T cells, % . | . | bLCV-specific CD4 T cells, % . | . | Absolute YFP+ and YFP- CD4 T cell counts, ×106/L . | . | |||
---|---|---|---|---|---|---|---|---|---|
Animal and time after transplantation, mo . | YFP+ . | YFP- . | YFP+ . | YFP- . | YFP+ . | YFP- . | |||
M00117 | |||||||||
6 | 2.55 | 1.82 | 0.89 | 0.68 | 83 | 941 | |||
M00227 | |||||||||
6 | 0.21 | 8.45 | 1.81 | 0.27 | 56 | 586 | |||
J00116 | |||||||||
16 | 3.03 | 3.18 | ND | ND | 82 | 1618 | |||
M00093 | |||||||||
3 | 0.75 | 0.02 | ND | ND | 137 | 532 | |||
5 | 0.34 | 1.05 | 1.19 | 0.43 | 143 | 334 | |||
6 | 0.47 | 2.84 | 1.56 | 0.75 | 192 | 578 | |||
15 | 1.01 | 2.11 | 0.29 | 0.34 | 659 | 1466 |
. | bCMV-specific CD4 T cells, % . | . | bLCV-specific CD4 T cells, % . | . | Absolute YFP+ and YFP- CD4 T cell counts, ×106/L . | . | |||
---|---|---|---|---|---|---|---|---|---|
Animal and time after transplantation, mo . | YFP+ . | YFP- . | YFP+ . | YFP- . | YFP+ . | YFP- . | |||
M00117 | |||||||||
6 | 2.55 | 1.82 | 0.89 | 0.68 | 83 | 941 | |||
M00227 | |||||||||
6 | 0.21 | 8.45 | 1.81 | 0.27 | 56 | 586 | |||
J00116 | |||||||||
16 | 3.03 | 3.18 | ND | ND | 82 | 1618 | |||
M00093 | |||||||||
3 | 0.75 | 0.02 | ND | ND | 137 | 532 | |||
5 | 0.34 | 1.05 | 1.19 | 0.43 | 143 | 334 | |||
6 | 0.47 | 2.84 | 1.56 | 0.75 | 192 | 578 | |||
15 | 1.01 | 2.11 | 0.29 | 0.34 | 659 | 1466 |
Percentages shown are averages of 2 independent experiments, except for EBV-specific cells in M00117, which were determined only once. Percentages were determined as percentage of IFN-γ+ YFP+ (YFP-) CD3+CD4+ cells in the stimulated sample minus percentage of IFN-γ+ YFP+ (YFP-) CD3+CD4+ cells in the negative control sample. ND indicates none detected.