T-cell lines from 12 donors showing donor HLA type, yield of pp65 peptide-specific IFNγ+ T cells, the pentadecapeptides eliciting responses, specific epitopes, and restricting HLA alleles
. | HLA type of donor . | . | . | . | . | T-cell response to CMV-pp65 peptide pool, total cell no. (% IFN+ T cells) . | . | . | Subsets on day 18, % . | . | . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| No. . | A . | B . | C . | DRB*1 . | DQB*1 . | Day 0 . | Day 18 . | Fold increase . | CD4 . | CD8 . | HLA restriction . | Immunogenic pentadecapeptide . | Sequence eliciting response . | ||||||
| 1 | 0201 | 3901 | 0702 | 0401 | 0302 | ND | ND | ND | ND | ND | A*0201 | 123, 124 | NLVPMVATV495-503 | ||||||
| 0301 | 1302 | 06BC | 07AD | 02MN | ND | ND | ND | ND | ND | NA | NA | NA | |||||||
| 2 | 0201 | 4402 | 05MN | 1101 | 03AF | 20 (0.18) | 49 (7.6) | 103.4 | 73 | 21 | A*0201 | 123, 124 | NLVPMVATV495-503 | ||||||
| 2402 | 3901 | 12NP | 1101 | 03AF | 20 (0.18) | 49 (7.6) | 103.4 | 73 | 21 | A*2402 | 85, 86 | QYDPVAALF341-349 | |||||||
| 3 | 0201 | 4002 | 0202 | 1101 | 03AF | 20 (0.15) | 79.5 (5.3) | 79.5 | 65 | 32 | A*0201 | 123, 124 | NLVPMVATV495-503 | ||||||
| 2402 | 27FFW | 0202 | 12AG | 03AF | 20 (0.15) | 79.5 (5.3) | 79.5 | 65 | 32 | A*2402 | 85, 86 | QYDPVAALF341-349 | |||||||
| 4 | 0201 | 40CCP | 0304 | 1302 | 06CUK | ND | ND | ND | ND | ND | A*0201 | 123, 124 | NLVPMVATV495-503 | ||||||
| 31AB | 1509 | 07ET | 0801 | 0402 | ND | ND | ND | ND | ND | NA | NA | NA | |||||||
| 5 | 0206 | 46AB | 1202 | 1501 | 0401 | 20 (0.20) | 48 (5.0) | 60 | 55 | 41 | A*0206 | 123, 124 | NLVPMVATV495-503 | ||||||
| 0207 | 1518 | 0801 | 0405 | 06WG | 20 (0.20) | 48 (5.0) | 60 | 55 | 41 | NA | NA | NA | |||||||
| 6 | 0201 | 1501 | 0300 | 1302 | 0302 | 20 (0.30) | 52 (7.94) | 68 | 66 | 31 | A*0201 | 123, 124 | NLVPMVATV495-503 | ||||||
| 0301 | 4001 | 0304 | 0401 | 0604 | 20 (0.30) | 52 (7.94) | 68 | 66 | 31 | B*4001 | 66, 67 | HERNGFTVL267-275 | |||||||
| 7 | 2402 | 27FFW | 0102 | 1501 | 06AAC | 20 (0.14) | 59 (4.52) | 95.2 | 55 | 41 | A*2402 | 85, 86 | QYDPVAALF341-349 | ||||||
| 68FPY | 27FFW | 0102 | 0402 | 06WG | 20 (0.14) | 59 (4.52) | 95.2 | 55 | 41 | NA | NA | NA | |||||||
| 8 | 6801 | 4002 | 04APA | 0804 | 03AF | 20 (0.15) | 62 (6.82) | 70.4 | 60 | 38 | B*4002 | 66, 67 | HERNGFTVL267-275 | ||||||
| 6601 | 5301 | 0305 | 1303 | 03AF | 20 (0.15) | 62 (6.82) | 70.4 | 60 | 38 | NA | NA | NA | |||||||
| 9 | 3002 | 4201 | 0202 | 0302 | 02MN | 20 (0.4) | 70 (5.9) | 51.6 | 55 | 42 | B*4201 | 66, 67 | RPHERNGFTVL265-275 | ||||||
| 3001 | 1503 | 17MN | 1101 | 0402 | 20 (0.4) | 70 (5.9) | 51.6 | 55 | 42 | DRB1*1101 | 91, 92 | EHPTFTSQYRI513-523 | |||||||
| 10 | 03AD | 0702 | 0702 | 0701 | 02XX | 20 (0.23) | 60 (4.8) | 62.6 | 65 | 32 | B*0702 | 66, 67 | RPHERNGFTV265-274 | ||||||
| 31AB | 0702 | 0702 | 1501 | 06XX | 20 (0.23) | 60 (4.8) | 62.6 | 65 | 32 | NA | NA | NA | |||||||
| 11 | 26JV | 44CU | 1601 | 1301 | 06AAC | 20 (0.2) | 56 (7.91) | 110 | 73 | 24 | DRB1*1301 | 128, 129 | FFWDANDIYRI513-523 | ||||||
| 29BD | 08DKG | 07AG | 1501 | 06WG | 20 (0.2) | 56 (7.91) | 110 | 73 | 24 | NA | NA | NA | |||||||
| 12 | 0101 | 4403 | 03XX | 0702 | 05XX | 20 (0.14) | 48 (4.7) | 75.2 | 75 | 22 | DQBI*05XX | 128, 129 | FFWDANDIYRI513-523 | ||||||
| 2301 | 5501 | 04XX | 1602 | 02XX | 20 (0.14) | 48 (4.7) | 75.2 | 75 | 22 | NA | NA | NA | |||||||
. | HLA type of donor . | . | . | . | . | T-cell response to CMV-pp65 peptide pool, total cell no. (% IFN+ T cells) . | . | . | Subsets on day 18, % . | . | . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| No. . | A . | B . | C . | DRB*1 . | DQB*1 . | Day 0 . | Day 18 . | Fold increase . | CD4 . | CD8 . | HLA restriction . | Immunogenic pentadecapeptide . | Sequence eliciting response . | ||||||
| 1 | 0201 | 3901 | 0702 | 0401 | 0302 | ND | ND | ND | ND | ND | A*0201 | 123, 124 | NLVPMVATV495-503 | ||||||
| 0301 | 1302 | 06BC | 07AD | 02MN | ND | ND | ND | ND | ND | NA | NA | NA | |||||||
| 2 | 0201 | 4402 | 05MN | 1101 | 03AF | 20 (0.18) | 49 (7.6) | 103.4 | 73 | 21 | A*0201 | 123, 124 | NLVPMVATV495-503 | ||||||
| 2402 | 3901 | 12NP | 1101 | 03AF | 20 (0.18) | 49 (7.6) | 103.4 | 73 | 21 | A*2402 | 85, 86 | QYDPVAALF341-349 | |||||||
| 3 | 0201 | 4002 | 0202 | 1101 | 03AF | 20 (0.15) | 79.5 (5.3) | 79.5 | 65 | 32 | A*0201 | 123, 124 | NLVPMVATV495-503 | ||||||
| 2402 | 27FFW | 0202 | 12AG | 03AF | 20 (0.15) | 79.5 (5.3) | 79.5 | 65 | 32 | A*2402 | 85, 86 | QYDPVAALF341-349 | |||||||
| 4 | 0201 | 40CCP | 0304 | 1302 | 06CUK | ND | ND | ND | ND | ND | A*0201 | 123, 124 | NLVPMVATV495-503 | ||||||
| 31AB | 1509 | 07ET | 0801 | 0402 | ND | ND | ND | ND | ND | NA | NA | NA | |||||||
| 5 | 0206 | 46AB | 1202 | 1501 | 0401 | 20 (0.20) | 48 (5.0) | 60 | 55 | 41 | A*0206 | 123, 124 | NLVPMVATV495-503 | ||||||
| 0207 | 1518 | 0801 | 0405 | 06WG | 20 (0.20) | 48 (5.0) | 60 | 55 | 41 | NA | NA | NA | |||||||
| 6 | 0201 | 1501 | 0300 | 1302 | 0302 | 20 (0.30) | 52 (7.94) | 68 | 66 | 31 | A*0201 | 123, 124 | NLVPMVATV495-503 | ||||||
| 0301 | 4001 | 0304 | 0401 | 0604 | 20 (0.30) | 52 (7.94) | 68 | 66 | 31 | B*4001 | 66, 67 | HERNGFTVL267-275 | |||||||
| 7 | 2402 | 27FFW | 0102 | 1501 | 06AAC | 20 (0.14) | 59 (4.52) | 95.2 | 55 | 41 | A*2402 | 85, 86 | QYDPVAALF341-349 | ||||||
| 68FPY | 27FFW | 0102 | 0402 | 06WG | 20 (0.14) | 59 (4.52) | 95.2 | 55 | 41 | NA | NA | NA | |||||||
| 8 | 6801 | 4002 | 04APA | 0804 | 03AF | 20 (0.15) | 62 (6.82) | 70.4 | 60 | 38 | B*4002 | 66, 67 | HERNGFTVL267-275 | ||||||
| 6601 | 5301 | 0305 | 1303 | 03AF | 20 (0.15) | 62 (6.82) | 70.4 | 60 | 38 | NA | NA | NA | |||||||
| 9 | 3002 | 4201 | 0202 | 0302 | 02MN | 20 (0.4) | 70 (5.9) | 51.6 | 55 | 42 | B*4201 | 66, 67 | RPHERNGFTVL265-275 | ||||||
| 3001 | 1503 | 17MN | 1101 | 0402 | 20 (0.4) | 70 (5.9) | 51.6 | 55 | 42 | DRB1*1101 | 91, 92 | EHPTFTSQYRI513-523 | |||||||
| 10 | 03AD | 0702 | 0702 | 0701 | 02XX | 20 (0.23) | 60 (4.8) | 62.6 | 65 | 32 | B*0702 | 66, 67 | RPHERNGFTV265-274 | ||||||
| 31AB | 0702 | 0702 | 1501 | 06XX | 20 (0.23) | 60 (4.8) | 62.6 | 65 | 32 | NA | NA | NA | |||||||
| 11 | 26JV | 44CU | 1601 | 1301 | 06AAC | 20 (0.2) | 56 (7.91) | 110 | 73 | 24 | DRB1*1301 | 128, 129 | FFWDANDIYRI513-523 | ||||||
| 29BD | 08DKG | 07AG | 1501 | 06WG | 20 (0.2) | 56 (7.91) | 110 | 73 | 24 | NA | NA | NA | |||||||
| 12 | 0101 | 4403 | 03XX | 0702 | 05XX | 20 (0.14) | 48 (4.7) | 75.2 | 75 | 22 | DQBI*05XX | 128, 129 | FFWDANDIYRI513-523 | ||||||
| 2301 | 5501 | 04XX | 1602 | 02XX | 20 (0.14) | 48 (4.7) | 75.2 | 75 | 22 | NA | NA | NA | |||||||
ND indicates not determined; NA, not applicable.