Sequences of primers used for amplifications of Pax-5, switch transcripts, Id-2, Blimp, and GAPDH genes
Genes . | Primer name . | Sequences 5′-3′ . |
|---|---|---|
| Pax-5 | ||
| 1) | For-Exon 1 (RT-PCR) | ATG GAT TTA GAG AAA AAT TAT CCG |
| 2) | For-Exon 3 (RT-PCR) | ATG TTT GCC TGG GAG ATC AGG G |
| 3) | For-Exon 5 (RT-PCR) | CGG CTC GTC GTA CTC CAT CAG |
| 4) | Rev-Exon 5 (RT-PCR) | GAC GTG ATG CCC AGG ATG CCG |
| 5) | Rev-Exon 8 (RT-PCR) | GCA CCA TCC CTG TCA GCG TCG |
| 6) | Rev-Exon 10 (RT-PCR) | GCC ATC AGT GTT TGG TGC CCG CC |
| 7) | Rev-Pax-5d/e (RT-PCR) | CGA AAA GGA CAG CGT GCG GGC |
| A) | For-Exon 7 (QRT-PCR) | CTG GAC CGC GTG TTT GAG AGG C |
| B) | Rev-Exon 8 (QRT-PCR) | CCG ACG CTG ACA GGG ATG GTG C |
| C) | Rev-Exon 7/9 (QRT-PCR) | CGG AAA ACT CAC TCC CTG TCA CAA TGG G |
| Switch | ||
| transcripts | ||
| μ-REV-CH1 (RT-PCR) | C TCT CAG GAC TGA TGG GAA GCC CCG | |
| δ-REV-CH1 (RT-PCR) | AGT CAC GGA CGT TGG GTG GTA CCC | |
| γ-REV-CH1 (RT-PCR) | GCT CTT GGA GGA GGG TGC CAG GGG G | |
| α-REV-CH1 (RT-PCR) | CTG TGT GGC CGG CAG GGT CAG CTG G | |
| Id-2 | ||
| For-ATG (RT-PCR) | AGC ATG AAA GCC TTC AGT CCC G | |
| Rev-Stop (RT-PCR) | CAT GAA CAC CGC TTA TTC AGC CAC | |
| For-LC (QRT-PCR) | GCA TCC CCC AGA ACA AGA AGG TG | |
| Rev-LC (QRT-PCR) | CAT GAA CAC CGC TTA TTC AGC CAC | |
| Prdm-1 | ||
| For-Exon 7 (QRT-PCR) | AGT GCA CAG TGG AGA ACG GCC | |
| Rev-Exon 8 (QRT-PCR) | CTT GTG GCA CTG GGA GCA C | |
| GAPDH | ||
| For (RT-PCR) | GGT GCT GAG TAT GTC GTG GA | |
| Rev (RT-PCR) | ATG CCA GTG AGC TTC CCG TT |
Genes . | Primer name . | Sequences 5′-3′ . |
|---|---|---|
| Pax-5 | ||
| 1) | For-Exon 1 (RT-PCR) | ATG GAT TTA GAG AAA AAT TAT CCG |
| 2) | For-Exon 3 (RT-PCR) | ATG TTT GCC TGG GAG ATC AGG G |
| 3) | For-Exon 5 (RT-PCR) | CGG CTC GTC GTA CTC CAT CAG |
| 4) | Rev-Exon 5 (RT-PCR) | GAC GTG ATG CCC AGG ATG CCG |
| 5) | Rev-Exon 8 (RT-PCR) | GCA CCA TCC CTG TCA GCG TCG |
| 6) | Rev-Exon 10 (RT-PCR) | GCC ATC AGT GTT TGG TGC CCG CC |
| 7) | Rev-Pax-5d/e (RT-PCR) | CGA AAA GGA CAG CGT GCG GGC |
| A) | For-Exon 7 (QRT-PCR) | CTG GAC CGC GTG TTT GAG AGG C |
| B) | Rev-Exon 8 (QRT-PCR) | CCG ACG CTG ACA GGG ATG GTG C |
| C) | Rev-Exon 7/9 (QRT-PCR) | CGG AAA ACT CAC TCC CTG TCA CAA TGG G |
| Switch | ||
| transcripts | ||
| μ-REV-CH1 (RT-PCR) | C TCT CAG GAC TGA TGG GAA GCC CCG | |
| δ-REV-CH1 (RT-PCR) | AGT CAC GGA CGT TGG GTG GTA CCC | |
| γ-REV-CH1 (RT-PCR) | GCT CTT GGA GGA GGG TGC CAG GGG G | |
| α-REV-CH1 (RT-PCR) | CTG TGT GGC CGG CAG GGT CAG CTG G | |
| Id-2 | ||
| For-ATG (RT-PCR) | AGC ATG AAA GCC TTC AGT CCC G | |
| Rev-Stop (RT-PCR) | CAT GAA CAC CGC TTA TTC AGC CAC | |
| For-LC (QRT-PCR) | GCA TCC CCC AGA ACA AGA AGG TG | |
| Rev-LC (QRT-PCR) | CAT GAA CAC CGC TTA TTC AGC CAC | |
| Prdm-1 | ||
| For-Exon 7 (QRT-PCR) | AGT GCA CAG TGG AGA ACG GCC | |
| Rev-Exon 8 (QRT-PCR) | CTT GTG GCA CTG GGA GCA C | |
| GAPDH | ||
| For (RT-PCR) | GGT GCT GAG TAT GTC GTG GA | |
| Rev (RT-PCR) | ATG CCA GTG AGC TTC CCG TT |
For indicates forward; rev, reverse; RT-PCR, primers used in reverse transcription-polymerase chain reaction; and QRT-PCR, quantitative real-time PCR.