Expression profile of AID, CSR, Pax-5, Id-2, and prdm-1 transcripts in CLL and normal B cells before and after CD40L + IL-4 stimulation
. | . | . | Pax-5, RT-PCR transcripts . | . | . | . | . | . | . | . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | AID, RT-PCR transcripts . | . | No . | . | Yes . | . | Id-2, RT-PCR transcripts . | . | prdm-1, QRT-PCR RU . | . | CSR, RT-PCR transcripts . | . | ||||||
| CD40+IL-4 stimulation, expression . | No . | Yes . | a . | Δ8 . | a . | Δ8 . | No . | Yes . | No . | Yes . | No . | Yes . | ||||||
| B-cell samples | ||||||||||||||||||
| CLL 1* | High | High | High | Neg | High | Neg | Low | Low | 8.5 | 3.2 | + | + | ||||||
| CLL 2* | High | High | High | Neg | High | Neg | Low | Low | 10.4 | 4.3 | + | + | ||||||
| CLL 3 | High | High | High | Neg | High | Neg | Low | Low | 7.3 | 4.2 | + | + | ||||||
| CLL 4 | High | High | High | Low | High | Neg | Low | Low | 8.6 | 5.6 | + | + | ||||||
| CLL 28 | Neg | High | Low | High | High | Neg | High | Low | 39.9 | 9.7 | - | + | ||||||
| CLL 29 | Neg | High | Low | Low | High | Neg | High | Low | 35.6 | 11 | - | + | ||||||
| CLL 30 | Neg | High | Low | High | High | Neg | High | Low | 38.8 | 9.9 | ND | ND | ||||||
| CLL 31 | Neg | High | Low | High | High | Neg | High | Low | 43.0 | 5.6 | - | + | ||||||
| CLL 32 | Neg | High | Low | Low | High | Neg | High | Low | 46.7 | 7.8 | - | + | ||||||
| Normal 55 | Neg | High | Low | Low | High | Neg | High | Neg | ND | ND | ND | ND | ||||||
| Normal 56 | Neg | High | Low | High | High | Neg | High | Low | ND | ND | ND | ND | ||||||
| Normal 57 | Neg | High | Low | Low | High | Neg | High | Low | ND | ND | ND | ND | ||||||
. | . | . | Pax-5, RT-PCR transcripts . | . | . | . | . | . | . | . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | AID, RT-PCR transcripts . | . | No . | . | Yes . | . | Id-2, RT-PCR transcripts . | . | prdm-1, QRT-PCR RU . | . | CSR, RT-PCR transcripts . | . | ||||||
| CD40+IL-4 stimulation, expression . | No . | Yes . | a . | Δ8 . | a . | Δ8 . | No . | Yes . | No . | Yes . | No . | Yes . | ||||||
| B-cell samples | ||||||||||||||||||
| CLL 1* | High | High | High | Neg | High | Neg | Low | Low | 8.5 | 3.2 | + | + | ||||||
| CLL 2* | High | High | High | Neg | High | Neg | Low | Low | 10.4 | 4.3 | + | + | ||||||
| CLL 3 | High | High | High | Neg | High | Neg | Low | Low | 7.3 | 4.2 | + | + | ||||||
| CLL 4 | High | High | High | Low | High | Neg | Low | Low | 8.6 | 5.6 | + | + | ||||||
| CLL 28 | Neg | High | Low | High | High | Neg | High | Low | 39.9 | 9.7 | - | + | ||||||
| CLL 29 | Neg | High | Low | Low | High | Neg | High | Low | 35.6 | 11 | - | + | ||||||
| CLL 30 | Neg | High | Low | High | High | Neg | High | Low | 38.8 | 9.9 | ND | ND | ||||||
| CLL 31 | Neg | High | Low | High | High | Neg | High | Low | 43.0 | 5.6 | - | + | ||||||
| CLL 32 | Neg | High | Low | Low | High | Neg | High | Low | 46.7 | 7.8 | - | + | ||||||
| Normal 55 | Neg | High | Low | Low | High | Neg | High | Neg | ND | ND | ND | ND | ||||||
| Normal 56 | Neg | High | Low | High | High | Neg | High | Low | ND | ND | ND | ND | ||||||
| Normal 57 | Neg | High | Low | Low | High | Neg | High | Low | ND | ND | ND | ND | ||||||
High and low refer to the ratio of a given transcript to GAPDH transcripts as assessed by semiquantitative RT-PCR.
RU indicates relative units of prdm-1/GAPDH by QRT-PCR; +, VDJ-Cγ and/or VDJ-Cα isotype presence; -, absence of isotype transcripts; ND, not determined.
Isotype switch was studied in these cases from purified, cell-sorted B cells