PCR primers
ANV reverse primer 1 | 5′-ATCAAGCTTATGCATGTCATCTTCTCCACAGAG-3′ |
| ANV forward primer 2 | 5′-GATCGGATCCAGTCTGGTCCTGCTTCACCTT-3′ |
| ANV-NdeI | 5′-GGAATTCCATATGGCACAGGTTCTCAGAGG-3′ |
| ANV-NsiI | 5′-CCAATGCATGTCATCTTCTCCAGC-3′ |
| 6L15-NsiI | 5′-CCAATGCATCCGGACTTCTGCCTG-3′ |
| KKTFPI-NsiI | 5′-CCAATGCATTCATTTTGTGCATTC-3′ |
| 6L15-SalI | 5′-ACGCGTCGACTTAAGCACCACCGCAAG-3′ |
| KKTFPI-SalI | 5′-ACGCGTCGACTTAGGTTCCATAATTATCC-3′ |
| TAP-NdeI | 5′-GGAATTCCATATGGCTTACAACCGTCTGTG-3′ |
| TAP-BamHI | 5′-CGGGATCCGATGCAAGCGTTGAAGCAG-3′ |
| ANV-BamHI | 5′-CGGGATCCGCACAGGTTCTCAGAGGC-3′ |
| ANV-SalI | 5′-ACGCGTCGACTTAGTCATCTTCTCCAGCG-3′ |
| ANV-XhoI | 5′-CCGCTCGAGAAAAGAGCACAGGTTCTCAGAG-3′ |
| KAPP-NotI | 5′-ATAAGAATGCGGCCGCTTAAATAGCAGAACCAC-3′ |
| ANV-EcoRV | 5′-CGCGATATCATCTTCTCCAGCGAG-3′ |
| 5′-KAPP | 5′-GAGGTTTGTTCTGAGCAAGC-3′ |
ANV reverse primer 1 | 5′-ATCAAGCTTATGCATGTCATCTTCTCCACAGAG-3′ |
| ANV forward primer 2 | 5′-GATCGGATCCAGTCTGGTCCTGCTTCACCTT-3′ |
| ANV-NdeI | 5′-GGAATTCCATATGGCACAGGTTCTCAGAGG-3′ |
| ANV-NsiI | 5′-CCAATGCATGTCATCTTCTCCAGC-3′ |
| 6L15-NsiI | 5′-CCAATGCATCCGGACTTCTGCCTG-3′ |
| KKTFPI-NsiI | 5′-CCAATGCATTCATTTTGTGCATTC-3′ |
| 6L15-SalI | 5′-ACGCGTCGACTTAAGCACCACCGCAAG-3′ |
| KKTFPI-SalI | 5′-ACGCGTCGACTTAGGTTCCATAATTATCC-3′ |
| TAP-NdeI | 5′-GGAATTCCATATGGCTTACAACCGTCTGTG-3′ |
| TAP-BamHI | 5′-CGGGATCCGATGCAAGCGTTGAAGCAG-3′ |
| ANV-BamHI | 5′-CGGGATCCGCACAGGTTCTCAGAGGC-3′ |
| ANV-SalI | 5′-ACGCGTCGACTTAGTCATCTTCTCCAGCG-3′ |
| ANV-XhoI | 5′-CCGCTCGAGAAAAGAGCACAGGTTCTCAGAG-3′ |
| KAPP-NotI | 5′-ATAAGAATGCGGCCGCTTAAATAGCAGAACCAC-3′ |
| ANV-EcoRV | 5′-CGCGATATCATCTTCTCCAGCGAG-3′ |
| 5′-KAPP | 5′-GAGGTTTGTTCTGAGCAAGC-3′ |
Restriction enzyme cleavage sites are underlined.