Effect of 8-Cl-Ado on the RNA inhibition
. | RNA synthesis, % mean . | . | . | ||
|---|---|---|---|---|---|
| Patient no. . | 0 h (dpm/106 cells) . | 2 h . | 4 h . | ||
| 1 | 100 (1360) | 67 | 40 | ||
| 2 | 100 (1502) | 60 | 35 | ||
| 3 | 100 (2805) | 57 | 32 | ||
| 4 | 100 (648) | 55 | 42 | ||
| 5 | 100 (3450) | 62 | 37 | ||
. | RNA synthesis, % mean . | . | . | ||
|---|---|---|---|---|---|
| Patient no. . | 0 h (dpm/106 cells) . | 2 h . | 4 h . | ||
| 1 | 100 (1360) | 67 | 40 | ||
| 2 | 100 (1502) | 60 | 35 | ||
| 3 | 100 (2805) | 57 | 32 | ||
| 4 | 100 (648) | 55 | 42 | ||
| 5 | 100 (3450) | 62 | 37 | ||
Primary CLL cells of patients 1 to 5 were isolated and the cells (5 × 106/500 μL) were incubated without drug or with 10 μM of 8-Cl-Ado for 2- and 4-hour periods and pulsed with [3H] uridine for 30 minutes. At the end of incubation the uridine incorporation into RNA was measured. The values are expressed as percentage of control (untreated) cells.