Effect of 10 μM 8-Cl-Ado on antiapoptotic proteins
. | % of control . | . | . | . | |||
|---|---|---|---|---|---|---|---|
| . | MCL-1 . | . | BCL-2 . | . | |||
| Patient no. . | 4 h . | 24 h . | 4 h . | 24 h . | |||
| 3 | 88 | 3 | 100 | 99 | |||
| 4 | 98 | 6 | 98 | 97 | |||
| 5 | 57 | 52 | 97 | 90 | |||
| 9 | 83 | ND | 96 | ND | |||
| 10 | 65 | 39 | 99 | 92 | |||
| 11 | 73 | 40 | 99 | 93 | |||
| 12 | 48 | 13 | 96 | 91 | |||
| 13 | 86 | 49 | ND | ND | |||
| 14 | 85 | 30 | ND | ND | |||
. | % of control . | . | . | . | |||
|---|---|---|---|---|---|---|---|
| . | MCL-1 . | . | BCL-2 . | . | |||
| Patient no. . | 4 h . | 24 h . | 4 h . | 24 h . | |||
| 3 | 88 | 3 | 100 | 99 | |||
| 4 | 98 | 6 | 98 | 97 | |||
| 5 | 57 | 52 | 97 | 90 | |||
| 9 | 83 | ND | 96 | ND | |||
| 10 | 65 | 39 | 99 | 92 | |||
| 11 | 73 | 40 | 99 | 93 | |||
| 12 | 48 | 13 | 96 | 91 | |||
| 13 | 86 | 49 | ND | ND | |||
| 14 | 85 | 30 | ND | ND | |||
Proteins were separated and immunoblotted for MCL-1 and BCL-2 and quantitated using a densitometer. The values are normalized based on the levels of actin and the value in untreated cells. Value in untreated cells is referred to as control, which is expressed as 100%.
ND indicates not done.