Influence of cytokines on the expression of CXCR3
Culture conditions . | CXCR3+ cell frequencies . | Change in CXCR3+ cells . | No. of experiments . |
|---|---|---|---|
| CD40L, IL-2, IL-10 (basal condition) | 19.5% ± 3.8% | 0 | 8 |
| +IFN-γ (200 U/mL) d 0 | 50.0% ± 6.5% | 30.5% ± 10.3% | 8 |
| +IFN-γ (2000 U/mL) d 0 | 65.8% ± 4.7% | 34.7% ± 8.5% | 3 |
| +IFN-γ (200 U/mL) d 3 | 12.8% ± 1.0% | 4.5% ± 4.8% | 3 |
| +IFN-α/β (20 U/mL) d 0 | 23.4% ± 1.8% | 3.9% ± 5.6% | 4 |
| +IFN-α/β (200 U/mL) d 0 | 18.4% ± 1.6% | -1.1% ± 5.4% | 4 |
| +IFN-α/β (6600 U/mL) d 0 | 24.1% ± 1.8% | 4.6% ± 5.6% | 4 |
| +IL-4 (100 U/mL) d 0 | 22.4% ± 5.5% | 3.7% ± 9.3% | 5 |
| +IL-6 (50 U/mL) d 0 | 22.9% ± 3.7% | -8.2% ± 7.5% | 3 |
| +IL-1β (50 U/mL) d 0 | 33.6% ± 0.7% | 0.6% ± 4.5% | 2 |
| +TNF-α (200 U/mL) d 0 | 33.1% ± 0.7% | -2.2% ± 4.5% | 2 |
| CpG 2006, IL-2, IL-10 | 25.8% ± 2.6% | 0 | 5 |
| +IFN-γ (200 U/mL) d 0 | 43.4% ± 2.3% | 30.5% ± 10.3% | 5 |
Culture conditions . | CXCR3+ cell frequencies . | Change in CXCR3+ cells . | No. of experiments . |
|---|---|---|---|
| CD40L, IL-2, IL-10 (basal condition) | 19.5% ± 3.8% | 0 | 8 |
| +IFN-γ (200 U/mL) d 0 | 50.0% ± 6.5% | 30.5% ± 10.3% | 8 |
| +IFN-γ (2000 U/mL) d 0 | 65.8% ± 4.7% | 34.7% ± 8.5% | 3 |
| +IFN-γ (200 U/mL) d 3 | 12.8% ± 1.0% | 4.5% ± 4.8% | 3 |
| +IFN-α/β (20 U/mL) d 0 | 23.4% ± 1.8% | 3.9% ± 5.6% | 4 |
| +IFN-α/β (200 U/mL) d 0 | 18.4% ± 1.6% | -1.1% ± 5.4% | 4 |
| +IFN-α/β (6600 U/mL) d 0 | 24.1% ± 1.8% | 4.6% ± 5.6% | 4 |
| +IL-4 (100 U/mL) d 0 | 22.4% ± 5.5% | 3.7% ± 9.3% | 5 |
| +IL-6 (50 U/mL) d 0 | 22.9% ± 3.7% | -8.2% ± 7.5% | 3 |
| +IL-1β (50 U/mL) d 0 | 33.6% ± 0.7% | 0.6% ± 4.5% | 2 |
| +TNF-α (200 U/mL) d 0 | 33.1% ± 0.7% | -2.2% ± 4.5% | 2 |
| CpG 2006, IL-2, IL-10 | 25.8% ± 2.6% | 0 | 5 |
| +IFN-γ (200 U/mL) d 0 | 43.4% ± 2.3% | 30.5% ± 10.3% | 5 |
Sorted CXCR3- peripheral blood B cells were stimulated with either CD40L-bearing EL4-B5 cells, IL-2, and IL-10, or with CpG 2006, IL-2, and IL-10 to induce differentiation into CD38++/CD20- plasma cells (basal stimulation condition). Additional cytokines were added at day 0 or at day 3 as indicated. This table shows the frequencies of CXCR3+ cells in individual cultures and the changes induced by the added cytokines compared to cells cultured under basal culture conditions in the same experiment. Results are mean values from repeated experiments.