Table 1.

Influence of MSCs on the immunophenotype of already differentiated DCs



Immature DCs

Mature DCs

LPS
LPS/MSC
MSC-negative
MSC-positive
Monocyte/macrophage antigen     
   CD14   2.9 ± 0.8   10.9 ± 3.0†   4.7 ± 1.5   2.8 ± 0.5  
Maturation antigen     
   CD83   78 ± 12.7   55 ± 15.6†   72.4 ± 4.2   39.3 ± 7.5‡  
Presentation molecules     
   HLA-DR   89.5 ± 9.2   77.5 ± 3.5†   81.5 ± 2.2   67.7 ± 8.5†  
   CD1a   72.4 ± 13.2   65.6 ± 10.7   55.0 ± 5.0   28.0 ± 6.1†  
Costimulatory molecules     
   CD86   91.0 ± 8.5   80.5 ± 9.2   89.6 ± 8.9   62.6 ± 17.1†  
   CD80   52 ± 7.1   45 ± 1.4   48.7 ± 7.4   14.9 ± 1.9‡  
Endocytosis     
   FITC-dextran*
 
63.5 ± 7.8
 
33.5 ± 17.7†
 
21.5 ± 3.9
 
22.9 ± 6.9
 


Immature DCs

Mature DCs

LPS
LPS/MSC
MSC-negative
MSC-positive
Monocyte/macrophage antigen     
   CD14   2.9 ± 0.8   10.9 ± 3.0†   4.7 ± 1.5   2.8 ± 0.5  
Maturation antigen     
   CD83   78 ± 12.7   55 ± 15.6†   72.4 ± 4.2   39.3 ± 7.5‡  
Presentation molecules     
   HLA-DR   89.5 ± 9.2   77.5 ± 3.5†   81.5 ± 2.2   67.7 ± 8.5†  
   CD1a   72.4 ± 13.2   65.6 ± 10.7   55.0 ± 5.0   28.0 ± 6.1†  
Costimulatory molecules     
   CD86   91.0 ± 8.5   80.5 ± 9.2   89.6 ± 8.9   62.6 ± 17.1†  
   CD80   52 ± 7.1   45 ± 1.4   48.7 ± 7.4   14.9 ± 1.9‡  
Endocytosis     
   FITC-dextran*
 
63.5 ± 7.8
 
33.5 ± 17.7†
 
21.5 ± 3.9
 
22.9 ± 6.9
 

Monocytes cultured with GM-CSF and IL-4 for 7 days were considered as immature DCs and, after stimulation by LPS for another 2 days, as mature DCs. The immunophenotype and the uptake of dextran were analyzed by FACS. Percentage of positive cells is shown as mean ± SD from 3 separate experiments (†P < .05, ‡P < .01).

*

The data are shown as mean fluorescence intensity

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