Influence of MSCs on the immunophenotype of already differentiated DCs
. | Immature DCs . | . | Mature DCs . | . | ||
|---|---|---|---|---|---|---|
. | LPS . | LPS/MSC . | MSC-negative . | MSC-positive . | ||
| Monocyte/macrophage antigen | ||||||
| CD14 | 2.9 ± 0.8 | 10.9 ± 3.0† | 4.7 ± 1.5 | 2.8 ± 0.5 | ||
| Maturation antigen | ||||||
| CD83 | 78 ± 12.7 | 55 ± 15.6† | 72.4 ± 4.2 | 39.3 ± 7.5‡ | ||
| Presentation molecules | ||||||
| HLA-DR | 89.5 ± 9.2 | 77.5 ± 3.5† | 81.5 ± 2.2 | 67.7 ± 8.5† | ||
| CD1a | 72.4 ± 13.2 | 65.6 ± 10.7 | 55.0 ± 5.0 | 28.0 ± 6.1† | ||
| Costimulatory molecules | ||||||
| CD86 | 91.0 ± 8.5 | 80.5 ± 9.2 | 89.6 ± 8.9 | 62.6 ± 17.1† | ||
| CD80 | 52 ± 7.1 | 45 ± 1.4 | 48.7 ± 7.4 | 14.9 ± 1.9‡ | ||
| Endocytosis | ||||||
| FITC-dextran* | 63.5 ± 7.8 | 33.5 ± 17.7† | 21.5 ± 3.9 | 22.9 ± 6.9 | ||
. | Immature DCs . | . | Mature DCs . | . | ||
|---|---|---|---|---|---|---|
. | LPS . | LPS/MSC . | MSC-negative . | MSC-positive . | ||
| Monocyte/macrophage antigen | ||||||
| CD14 | 2.9 ± 0.8 | 10.9 ± 3.0† | 4.7 ± 1.5 | 2.8 ± 0.5 | ||
| Maturation antigen | ||||||
| CD83 | 78 ± 12.7 | 55 ± 15.6† | 72.4 ± 4.2 | 39.3 ± 7.5‡ | ||
| Presentation molecules | ||||||
| HLA-DR | 89.5 ± 9.2 | 77.5 ± 3.5† | 81.5 ± 2.2 | 67.7 ± 8.5† | ||
| CD1a | 72.4 ± 13.2 | 65.6 ± 10.7 | 55.0 ± 5.0 | 28.0 ± 6.1† | ||
| Costimulatory molecules | ||||||
| CD86 | 91.0 ± 8.5 | 80.5 ± 9.2 | 89.6 ± 8.9 | 62.6 ± 17.1† | ||
| CD80 | 52 ± 7.1 | 45 ± 1.4 | 48.7 ± 7.4 | 14.9 ± 1.9‡ | ||
| Endocytosis | ||||||
| FITC-dextran* | 63.5 ± 7.8 | 33.5 ± 17.7† | 21.5 ± 3.9 | 22.9 ± 6.9 | ||
Monocytes cultured with GM-CSF and IL-4 for 7 days were considered as immature DCs and, after stimulation by LPS for another 2 days, as mature DCs. The immunophenotype and the uptake of dextran were analyzed by FACS. Percentage of positive cells is shown as mean ± SD from 3 separate experiments (†P < .05, ‡P < .01).
The data are shown as mean fluorescence intensity