Reagents and conditions used for immunohistochemistry
Antibody . | Source . | Dilution . | Pretreatment . | Predominant cell reactivity . | Staining pattern . |
|---|---|---|---|---|---|
| HGAL | * | 1:20 | Citrate | Lymphoid cells | Cytoplasmic |
| BCL6 | 1 | 1:40 | Tris | Lymphoid cells | Nuclear |
| CD10 | 2 | 1:40 | EDTA | Lymphoid cells | Membrane and cytoplasmic |
| MUM1/IRF4 | 1 | 1:50 | EDTA | Lymphoid cells | Nuclear and cytoplasmic |
| BCL2 | 1 | 1:1000 | Tris | Various | Cytoplasmic |
Antibody . | Source . | Dilution . | Pretreatment . | Predominant cell reactivity . | Staining pattern . |
|---|---|---|---|---|---|
| HGAL | * | 1:20 | Citrate | Lymphoid cells | Cytoplasmic |
| BCL6 | 1 | 1:40 | Tris | Lymphoid cells | Nuclear |
| CD10 | 2 | 1:40 | EDTA | Lymphoid cells | Membrane and cytoplasmic |
| MUM1/IRF4 | 1 | 1:50 | EDTA | Lymphoid cells | Nuclear and cytoplasmic |
| BCL2 | 1 | 1:1000 | Tris | Various | Cytoplasmic |
Generation of HGAL monoclonal antibody is described in “Materials and methods.” Source 1 was Dako; 2 was Novocastra (New Castle-upon-Tyne, United Kingdom). Pretreatment for heat-induced antigen retrieval consisted of microwaving with one of the following buffers: citrate (10 mM, pH 6.0, for 10 minutes), Tris (5 mM, pH 10.0 for 20 minutes), or EDTA (1 mM pH 8.0 for 15 minutes)