Table 2. Kinetic and stability... | American Society of Hematology

Table 2.

Kinetic and stability parameters of recombinant wild-type and mutant P5′N-1

	CMP					UMP
	K_cat, S⁻¹	K_m, mM	K_cat/K_m, s⁻¹mM⁻¹	K_cat, s⁻¹	K_m, mM	K_cat/K_m, s⁻¹mM⁻¹	T_50, °C
Wild type	26.14 ± 0.70	0.18 ± 0.02	145.00	11.98 ± 0.30	0.056 ± 0.006	214.00	52
D87V	23.30 ± 1.20	1.00 ± 0.10	23.30	14.27 ± 0.40	1.640 ± 0.100	8.90	42
L131P	6.39 ± 0.20	0.50 ± 0.07	13.07	6.86 ± 0.10	0.270 ± 0.020	25.40	37
N179S	0.93 ± 0.03	2.60 ± 0.20	0.36	0.27 ± 0.01	4.620 ± 0.500	0.06	51
G230R	0.24 ± 0.03	0.11 ± 0.02	4.00	0.06 ± 0.01	0.100 ± 0.010	0.60	45

	CMP					UMP
	K_cat, S⁻¹	K_m, mM	K_cat/K_m, s⁻¹mM⁻¹	K_cat, s⁻¹	K_m, mM	K_cat/K_m, s⁻¹mM⁻¹	T_50, °C
Wild type	26.14 ± 0.70	0.18 ± 0.02	145.00	11.98 ± 0.30	0.056 ± 0.006	214.00	52
D87V	23.30 ± 1.20	1.00 ± 0.10	23.30	14.27 ± 0.40	1.640 ± 0.100	8.90	42
L131P	6.39 ± 0.20	0.50 ± 0.07	13.07	6.86 ± 0.10	0.270 ± 0.020	25.40	37
N179S	0.93 ± 0.03	2.60 ± 0.20	0.36	0.27 ± 0.01	4.620 ± 0.500	0.06	51
G230R	0.24 ± 0.03	0.11 ± 0.02	4.00	0.06 ± 0.01	0.100 ± 0.010	0.60	45

Results are means (SE) for 3 determinations from at least 2 different protein preparations.