Table 1. Polymerization of fibrinogen... | American Society of Hematology

Table 1.

Polymerization of fibrinogen Philadelphia and clot structure as characterized by turbidity and electron microscopy

			Maximum rate, × 10^-5 sec^-1			Turbidity, optical density at 350 nm											Fiber diameter, nm
Experimental condition	Lag period, min		Maximum rate, × 10^-5 sec^-1					Control					Philadelphia					Control	Philadelphia
Experimental condition		Control	Phil	Control	Phil	2 min	10 min	30 min	2 min	10 min	30 min	2 min	10 min	30 min	2 min	10 min	30 min
0.20 M Ca⁺⁺	1.1 ± 0.3^*	0.6 ± 0.2^*	140 ± 18^*	180 ± 23^*	0.10 ± 0.05^*	0.73 ± 0.08^*	0.90 ± 0.09^*	0.19 ± 0.07^*	0.64 ± 0.09^*	0.75 ± 0.08^*	56 ± 14†	75 ± 27^*†	111 ± 21^*‡	57 ± 15†	62 ± 20^*†	91 ± 19^*‡
0.20 M citrate	0.8 ± 0.2	0.8 ± 0.2	25 ± 5^*	17 ± 4^*	0.02 ± 0.01	0.20 ± 0.06^*	0.24 ± 0.07^*	0.02 ± 0.01	0.11 ± 0.05^*	0.16 ± 0.06^*	—	41 ± 11†§	—	—	45 ± 11†§	—
0.4 M Ca⁺⁺	0	0	5 ± 1^*	1 ± 0.5^*	0.015 ± 0.003§	0.035 ± 0.010^*	—	0.018 ± 0.004§	0.020 ± 0.009^*	—	—	54 ± 15^*†	—	—	48 ± 14^*†	—
0.05 M citrate	0.6 ± 0.2^*	0.3 ± 0.2^*	140 ± 20	140 ± 17	0.13 ± 0.07^*	0.74 ± 0.10	0.98 ± 0.10	0.21 ± 0.08^*	0.72 ± 0.09	0.98 ± 0.11	—	—	—	—	—	—

			Maximum rate, × 10^-5 sec^-1			Turbidity, optical density at 350 nm											Fiber diameter, nm
Experimental condition	Lag period, min		Maximum rate, × 10^-5 sec^-1					Control					Philadelphia					Control	Philadelphia
Experimental condition		Control	Phil	Control	Phil	2 min	10 min	30 min	2 min	10 min	30 min	2 min	10 min	30 min	2 min	10 min	30 min
0.20 M Ca⁺⁺	1.1 ± 0.3^*	0.6 ± 0.2^*	140 ± 18^*	180 ± 23^*	0.10 ± 0.05^*	0.73 ± 0.08^*	0.90 ± 0.09^*	0.19 ± 0.07^*	0.64 ± 0.09^*	0.75 ± 0.08^*	56 ± 14†	75 ± 27^*†	111 ± 21^*‡	57 ± 15†	62 ± 20^*†	91 ± 19^*‡
0.20 M citrate	0.8 ± 0.2	0.8 ± 0.2	25 ± 5^*	17 ± 4^*	0.02 ± 0.01	0.20 ± 0.06^*	0.24 ± 0.07^*	0.02 ± 0.01	0.11 ± 0.05^*	0.16 ± 0.06^*	—	41 ± 11†§	—	—	45 ± 11†§	—
0.4 M Ca⁺⁺	0	0	5 ± 1^*	1 ± 0.5^*	0.015 ± 0.003§	0.035 ± 0.010^*	—	0.018 ± 0.004§	0.020 ± 0.009^*	—	—	54 ± 15^*†	—	—	48 ± 14^*†	—
0.05 M citrate	0.6 ± 0.2^*	0.3 ± 0.2^*	140 ± 20	140 ± 17	0.13 ± 0.07^*	0.74 ± 0.10	0.98 ± 0.10	0.21 ± 0.08^*	0.72 ± 0.09	0.98 ± 0.11	—	—	—	—	—	—

For turbidity and fiber diameters, the numbers of observations were 10 and 500, respectively.

Phil indicates fibrinogen Philadelphia; —, not determined.

P < .0001 versus control.

P < .001 versus control.

Indicates fiber diameter measurements from transmission electron micrographs of negatively contrasted fibrin.

Indicates fiber diameter measurements from scanning electron micrographs of fibrin clots.