Comparison of chemotactic and chemokinetic activities of FL and CXCL12 in Ba/F3-Flt3 cells
. | FL . | CXCL12 . |
|---|---|---|
| MCTA, % | 2.2 | 20.2 |
| MCKA, % | 1.9 | 0.04 |
| CCI | 1.1 | 505 |
| RCKA | 89.9 | 0.2 |
. | FL . | CXCL12 . |
|---|---|---|
| MCTA, % | 2.2 | 20.2 |
| MCKA, % | 1.9 | 0.04 |
| CCI | 1.1 | 505 |
| RCKA | 89.9 | 0.2 |
All calculations were performed based on the checkerboard assay (0-100 ng/mL) as described.11 The maximal response for migration was observed at 10 ng/mL and 100 ng/mL for FL and CXCL12, respectively.
MCTA indicates maximum chemotactic activity (a maximal percentage migration to the lower chamber in the positive gradient of the chemoattractant); MCKA, maximum chemokinetic activity (a maximal percentage migration to the lower chamber in the zero gradient formed by equal concentrations of the chemoattractant in both chambers); CCI, chemotactic-chemokinetic index (calculated by dividing MCTA by MCKA); and RCKA, relative chemokinetic activity (calculated by the following formula: [MCKA/MCTA] × 100).