Cytogenetic and hematologic characteristics of mice that received transplants of ex vivo-cultured BM cells
Donor cell genotype and mouse no. . | Culture time, d . | Karyotype* . | BM cellularity, × 106 LDMNCs . | Spleen weight, mg . | TNF-α sensitivity . | BM and spleen histology . | BM and spleen histology from secondary BMT . |
|---|---|---|---|---|---|---|---|
| +/+ | |||||||
| a-f | 0,2,4 | Normal | 16.8-22.8 | 91-150 | R | Normal | Normal |
| -/- | |||||||
| 1-6 | 0 | Normal | 18.4-22.4 | 97-160 | S | Normal | Normal |
| 7 | 0 | 40,X, - X or - Y, +6(30%)/39,XX,t(8;9)(C3;F4), - 14(10%) | 16.3 | 286 | S | Reactive proliferation† | Normal |
| 8 | 2 | Normal | 18.5 | 167 | S | Hypoplasia | Hypoplasia |
| 9 | 2 | 40,XX,t(4;17)(D3;E4)(10%)/40,XX,del(6)(DF3)(10%)/38,X, - X or - Y,t(4;9)(E1;F1), - 9(10%)/40,XY,der(15)t(15;16)(E;D)(10%) | 17.0 | 224 | R | Myeloproliferative disease | MDS |
| 10 | 2 | 40,XY,t(8;17)(D1;A2)(20%) | 12.7 | 358 | R | Myeloproliferative disease | Acute myeloid leukemia |
| 11 | 4 | Normal | 9.0 | 83 | S | Hypoplasia | Hypoplasia |
| 12 | 4 | 39,X, - X or - Y(30%) | 6.1 | 62 | R | Hypoplasia | MDS |
| 13 | 4 | 39,XY, - 13der(19)t(13;19)(B;D2)(10%) | 11.4 | 120 | S | Hypoplasia | NA‡ |
| 14 | 4 | 40,XY, - 4, +mar(10%) | 7.0 | 99 | R | Hypoplasia | Hypoplasia |
Donor cell genotype and mouse no. . | Culture time, d . | Karyotype* . | BM cellularity, × 106 LDMNCs . | Spleen weight, mg . | TNF-α sensitivity . | BM and spleen histology . | BM and spleen histology from secondary BMT . |
|---|---|---|---|---|---|---|---|
| +/+ | |||||||
| a-f | 0,2,4 | Normal | 16.8-22.8 | 91-150 | R | Normal | Normal |
| -/- | |||||||
| 1-6 | 0 | Normal | 18.4-22.4 | 97-160 | S | Normal | Normal |
| 7 | 0 | 40,X, - X or - Y, +6(30%)/39,XX,t(8;9)(C3;F4), - 14(10%) | 16.3 | 286 | S | Reactive proliferation† | Normal |
| 8 | 2 | Normal | 18.5 | 167 | S | Hypoplasia | Hypoplasia |
| 9 | 2 | 40,XX,t(4;17)(D3;E4)(10%)/40,XX,del(6)(DF3)(10%)/38,X, - X or - Y,t(4;9)(E1;F1), - 9(10%)/40,XY,der(15)t(15;16)(E;D)(10%) | 17.0 | 224 | R | Myeloproliferative disease | MDS |
| 10 | 2 | 40,XY,t(8;17)(D1;A2)(20%) | 12.7 | 358 | R | Myeloproliferative disease | Acute myeloid leukemia |
| 11 | 4 | Normal | 9.0 | 83 | S | Hypoplasia | Hypoplasia |
| 12 | 4 | 39,X, - X or - Y(30%) | 6.1 | 62 | R | Hypoplasia | MDS |
| 13 | 4 | 39,XY, - 13der(19)t(13;19)(B;D2)(10%) | 11.4 | 120 | S | Hypoplasia | NA‡ |
| 14 | 4 | 40,XY, - 4, +mar(10%) | 7.0 | 99 | R | Hypoplasia | Hypoplasia |
R indicates Fancc-/- myeloid progenitors that had < 10% reduction in maximal clonogenic numbers when cultured in the presence of 1 ng/mL of TNF-α, S,Fancc-/- myeloid progenitors that had ≤ 50% reduction in maximal clonogenic numbers when cultured in the presence of 1 ng/mL of TNF-α, and NA, not available.
Ten metaphase cells were analyzed from each sample; only cells with abnormal karyotypes are listed
This mouse had an eye infection at the time that it was killed
Recipient died unexpectedly after transplantation