Primer sequences
Name . | Accession no. . | First-strand primer . | PCR primer downstream . | PCR primer upstream . |
|---|---|---|---|---|
| Rap1GAP1a/Rap1GAP | M64788 | AGCCTGCACCACGACGTA | GCACAGGATAGAACCGATC | CATTCCATACCCGAGCGT |
| Rap1GAPIb/Rap1GAPII | AB003930 | AGCCTGCACCACGACGTA | GCACAGGATAGAACCGATC | CTGGCAGAACACAGATCTAT |
| SPA-1 | AB005666 | GCAATTGAACACTACGCG | GTAGTGGTCACCTCGTTG | CTAGACACCAAAACGGATT |
| E6TP1α | AF090989 | TCTCTGTCTCCAAAGTCCGAT | GAACCAGTATCACTGGATGAA | CCTCAAAATCTTCTATGAACG |
| Tuberin | X75621 | GAAGTTGGAGAAGACGTATCG | CATGTAGTCGCAGACGCAGTA | TGGTGTCCTATGAGATCGTCC |
| KIAA1039 | AB028962 | GCGGAATTTCAGGTTCGA | CATGGAGTGGCTGCGTAC | AGCTGCCATTTACCGACG |
| KIAA1389 | AB037810 | TACTCCTGCCTCGTTCGA | TTGTCTAGCTGAGCTCGATA | TGTCCCTCAATACCGTGC |
| KIAA0545 | AB011117 | TGGGTGCTTATTGCGTAC | TGGGTGCTTATTGCGTAC | GCTGCTCTTTCGAATCGT |
| p761J1523 (DKFZ) | AL136573 | CATCAAATTGGTGTCGATAA | CATATCCAGGGTACGCCG | TGTCCCTCAATACCGTGC |
| CD-GEFI | AF081194 | TGGAAGTTGTGTACGAAGC | TCCACTCCTCCAATACCG | CAGGACTATCACAGTTTCGT |
| CD-GEFIII | AB020653 | CCTCACAGAGATCTTGCG | CAAAGGCCTTGCGGTAATTG | CCTTGGAAAGATCGATTGCT |
| DOCK4 | AY233380 | TTGAAGAGCTCGCGGTAT | GTCTATCTGTCATTTGTCGTAAT | ATGGTACGTGGACCGGAT |
| Epac | AF103905 | GAGGGCCAGTCGGTATAC | CTCCAGTCGTGGTCCGTC | GAGGCAGCAGATCTTGCG |
| Epac2 | U78516 | TGAAATGTCTATGGTCGACG | AAACTTTCAAACTCCGCATAG | ATGTTTCAGTATTTACGACGC |
| Repac | D87467 | GGTCTGCGACAGTCGACT | CCAGGTCTTTCCGGTAGA | TCAGATGTTCCGCGTAGG |
| PDZ-GEF1 | AB002311 | AGAAAGGAACTACGACGTAC | TGGAATGGAGTAGCGACT | AGGCTGTTGAATATCGCG |
| PDZ-GEF2 | AF478567 | TCCAATACTCTCCGTTTAAC | CAGTGACCTCTGTCGAAAC | AAATTGCACTTCATTGTCGAGA |
| C3G | D21239 | CATTGAAGAAGTTTATAATGTCG | ATGGCTGTGAAAGTCGTGC | ATTCAAGAAGCGCGTCAGC |
| smg GDS | X63465 | GCGAAGTTTGGATACGAC | TTTTGATTTACTGTGTCGTATAAG | TGTTACGATAGCCATTCGCT |
Name . | Accession no. . | First-strand primer . | PCR primer downstream . | PCR primer upstream . |
|---|---|---|---|---|
| Rap1GAP1a/Rap1GAP | M64788 | AGCCTGCACCACGACGTA | GCACAGGATAGAACCGATC | CATTCCATACCCGAGCGT |
| Rap1GAPIb/Rap1GAPII | AB003930 | AGCCTGCACCACGACGTA | GCACAGGATAGAACCGATC | CTGGCAGAACACAGATCTAT |
| SPA-1 | AB005666 | GCAATTGAACACTACGCG | GTAGTGGTCACCTCGTTG | CTAGACACCAAAACGGATT |
| E6TP1α | AF090989 | TCTCTGTCTCCAAAGTCCGAT | GAACCAGTATCACTGGATGAA | CCTCAAAATCTTCTATGAACG |
| Tuberin | X75621 | GAAGTTGGAGAAGACGTATCG | CATGTAGTCGCAGACGCAGTA | TGGTGTCCTATGAGATCGTCC |
| KIAA1039 | AB028962 | GCGGAATTTCAGGTTCGA | CATGGAGTGGCTGCGTAC | AGCTGCCATTTACCGACG |
| KIAA1389 | AB037810 | TACTCCTGCCTCGTTCGA | TTGTCTAGCTGAGCTCGATA | TGTCCCTCAATACCGTGC |
| KIAA0545 | AB011117 | TGGGTGCTTATTGCGTAC | TGGGTGCTTATTGCGTAC | GCTGCTCTTTCGAATCGT |
| p761J1523 (DKFZ) | AL136573 | CATCAAATTGGTGTCGATAA | CATATCCAGGGTACGCCG | TGTCCCTCAATACCGTGC |
| CD-GEFI | AF081194 | TGGAAGTTGTGTACGAAGC | TCCACTCCTCCAATACCG | CAGGACTATCACAGTTTCGT |
| CD-GEFIII | AB020653 | CCTCACAGAGATCTTGCG | CAAAGGCCTTGCGGTAATTG | CCTTGGAAAGATCGATTGCT |
| DOCK4 | AY233380 | TTGAAGAGCTCGCGGTAT | GTCTATCTGTCATTTGTCGTAAT | ATGGTACGTGGACCGGAT |
| Epac | AF103905 | GAGGGCCAGTCGGTATAC | CTCCAGTCGTGGTCCGTC | GAGGCAGCAGATCTTGCG |
| Epac2 | U78516 | TGAAATGTCTATGGTCGACG | AAACTTTCAAACTCCGCATAG | ATGTTTCAGTATTTACGACGC |
| Repac | D87467 | GGTCTGCGACAGTCGACT | CCAGGTCTTTCCGGTAGA | TCAGATGTTCCGCGTAGG |
| PDZ-GEF1 | AB002311 | AGAAAGGAACTACGACGTAC | TGGAATGGAGTAGCGACT | AGGCTGTTGAATATCGCG |
| PDZ-GEF2 | AF478567 | TCCAATACTCTCCGTTTAAC | CAGTGACCTCTGTCGAAAC | AAATTGCACTTCATTGTCGAGA |
| C3G | D21239 | CATTGAAGAAGTTTATAATGTCG | ATGGCTGTGAAAGTCGTGC | ATTCAAGAAGCGCGTCAGC |
| smg GDS | X63465 | GCGAAGTTTGGATACGAC | TTTTGATTTACTGTGTCGTATAAG | TGTTACGATAGCCATTCGCT |
All primers are in 5′ to 3′ orientation.