ICOS−/− recipients have reduced graft-rejecting capacity
. | . | 6 wk . | . | 4 mo . | . | ||
|---|---|---|---|---|---|---|---|
| Donor . | Host . | No. chimeric . | % Donor . | No. chimeric . | % Donor . | ||
| BALB/c | B6 WT | 11/20 | 29 ± 6 | 7/15 | 32 ± 11 | ||
| BALB/c | B6 ICOS−/− | 20/20* | 61 ± 5* | 14/14* | 90 ± 4* | ||
. | . | 6 wk . | . | 4 mo . | . | ||
|---|---|---|---|---|---|---|---|
| Donor . | Host . | No. chimeric . | % Donor . | No. chimeric . | % Donor . | ||
| BALB/c | B6 WT | 11/20 | 29 ± 6 | 7/15 | 32 ± 11 | ||
| BALB/c | B6 ICOS−/− | 20/20* | 61 ± 5* | 14/14* | 90 ± 4* | ||
Host mice were sublethally irradiated on day 1 (5.5 Gy TBI) and infused with 10 × 106 allogeneic TCD donor BM on day 0. PBLs were typed for percentage donor-host at 6 weeks and 4 months after BMT. Chimeric is defined as having more than 3% donor PBLs. Percentage donor is defined as average percentage donor cells of all mice in the group ± 1 SEM.
P < .001 compared with B6 WT (pool of 2 experiments).