Colony-forming cell content of blast colonies
. | No. of colony-forming cells . | . | . | . | . | . | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Recloning stimulus . | Blast . | G . | GM . | M . | Eo . | Meg . | |||||
| M-CSF | |||||||||||
| Mpl+/+c-MybPlt4/Plt4 | 0 | 0.4 ± 1.8 | 0.8 ± 3.0 | 90 ± 168 | 0 | 0 | |||||
| Mpl+/+c-Myb+/+ | 0 | 15 ± 30 | 29 ± 42 | 826 ± 983 | 0 | 0 | |||||
| S3E | |||||||||||
| Mpl+/+c-Myblt4/lt4 | 0.4 ± 1.8 | 3.4 ± 7.9 | 2.4 ± 6.6 | 14 ± 35 | 0.2 ± 1.3 | 0 | |||||
| Mpl+/+c-Myb+/+ | 0.4 ± 1.8 | 14 ± 23 | 86 ± 135 | 180 ± 210 | 0.5 ± 1.9 | 1.3 ± 4.3 | |||||
| SCF + G-CSF | |||||||||||
| Mpl+/+c-MybPlt4/Plt4 | 0 | 0 | 0 | 0 | 0 | 0 | |||||
| Mpl+/+c-Myb+/+ | 0 | 55 ± 77 | 14 ± 43 | 11 ± 23 | 0 | 0 | |||||
. | No. of colony-forming cells . | . | . | . | . | . | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Recloning stimulus . | Blast . | G . | GM . | M . | Eo . | Meg . | |||||
| M-CSF | |||||||||||
| Mpl+/+c-MybPlt4/Plt4 | 0 | 0.4 ± 1.8 | 0.8 ± 3.0 | 90 ± 168 | 0 | 0 | |||||
| Mpl+/+c-Myb+/+ | 0 | 15 ± 30 | 29 ± 42 | 826 ± 983 | 0 | 0 | |||||
| S3E | |||||||||||
| Mpl+/+c-Myblt4/lt4 | 0.4 ± 1.8 | 3.4 ± 7.9 | 2.4 ± 6.6 | 14 ± 35 | 0.2 ± 1.3 | 0 | |||||
| Mpl+/+c-Myb+/+ | 0.4 ± 1.8 | 14 ± 23 | 86 ± 135 | 180 ± 210 | 0.5 ± 1.9 | 1.3 ± 4.3 | |||||
| SCF + G-CSF | |||||||||||
| Mpl+/+c-MybPlt4/Plt4 | 0 | 0 | 0 | 0 | 0 | 0 | |||||
| Mpl+/+c-Myb+/+ | 0 | 55 ± 77 | 14 ± 43 | 11 ± 23 | 0 | 0 | |||||
Mean values ± standard deviations of calculated total colony-forming cells per colony in secondary cultures of 40 primary blast colonies of each genotype.
G indicates granulocyte; GM, granulocyte-macrophage; M, macrophage; Eo, eosinophil, Meg, megakaryocyte; S3E, SCF + IL-3 + EPO.