Up-regulation of CD80 and CD83 expression during HCV-LP-induced DC activation
. | Δ MFI, mean ± SD . | . | |
|---|---|---|---|
. | CD80 . | CD83 . | |
| GUS Ctrl | 10 ± 8* | 7 ± 6* | |
| Native HCV-LPs | 41 ± 9*† | 48 ± 19*† | |
| Denatured HCV-LPs | 5.6 ± 4† | 0 ± 0† | |
| LPS | 140 ± 6 | 157 ± 21 | |
. | Δ MFI, mean ± SD . | . | |
|---|---|---|---|
. | CD80 . | CD83 . | |
| GUS Ctrl | 10 ± 8* | 7 ± 6* | |
| Native HCV-LPs | 41 ± 9*† | 48 ± 19*† | |
| Denatured HCV-LPs | 5.6 ± 4† | 0 ± 0† | |
| LPS | 140 ± 6 | 157 ± 21 | |
ImDCs were incubated with insect cell control preparation (GUS Ctrl), native HCV-LPs, denatured HCV-LPs, and lipopolysaccharide (LPS) as described in Figure 7. Cell surface expression of CD80 and CD83 was determined by flow cytometry. Δ MFI (net mean fluorescence intensity) was calculated by subtracting the MFI of DCs incubated without antigen (background) from the MFI of DCs incubated with the respective antigen. Mean ± SD of 3 independent experiments using imDCs from 3 different individuals is shown.
Differences in expression of DC activation markers between native HCV-LPs versus GUS Ctrl-treated DCs (*) as well as DCs incubated with native HCV-LPs versus denatured HCV-LPs (†) were statistically significant (P < .05; two-sided Student ttest).