Table 1.

Cytotoxicity of IL-4 cytotoxin to Hodgkin/Reed-Sternberg cells


Cell line

IC50, ng/mL

No. experiments performed

IL-4Rα mRNA expression status
L428   120-200   5   +  
L1236   0.1-0.5   8   ++  
L540   10-130   5   ++  
L591   2-20   6   ++  
HD-MyZ   2-35   7   ++  
HD-LM2
 
More than 1000
 
3
 
+
 

Cell line

IC50, ng/mL

No. experiments performed

IL-4Rα mRNA expression status
L428   120-200   5   +  
L1236   0.1-0.5   8   ++  
L540   10-130   5   ++  
L591   2-20   6   ++  
HD-MyZ   2-35   7   ++  
HD-LM2
 
More than 1000
 
3
 
+
 

IC50 is the concentration of IL4 -PE38KDEL at which 50% inhibition of protein synthesis was observed compared with untreated cells.

IL-4Rα mRNA expression was confirmed by RT-PCR analysis, as previously described.29  Band intensity was assigned + for faint band and ++ for strong band.

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