Repertoire analysis of CTL lines generated from 3 representative donors
. | CTLs, donor 1 . | . | . | CTLs, donor 2 . | . | . | CTLs, donor 3 . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Vβ . | NT . | EV or CsiRNA . | SiRNA10 . | NT . | EV or CsiRNA . | SiRNA10 . | NT . | EV or CsiRNA . | SiRNA10 . | ||||||
| Vβ1 | 1.66 | 0.98 | 1.43 | 5.71 | 4.38 | 4.66 | 7.48 | 9.67 | 5.14 | ||||||
| Vβ2 | 1.54 | 1.81 | 1.54 | 4.55 | 4.59 | 4.59 | 8.05 | 7.61 | 8.24 | ||||||
| Vβ3 | 2.49 | 2.02 | 2.2 | 0.58 | 1.15 | 0.83 | 7.67 | 11.18 | 8.21 | ||||||
| Vβ4 | 0.01 | 0.06 | 0.06 | 0.11 | 0.32 | 0.16 | 0.03 | 0.06 | 0.02 | ||||||
| Vβ5.1 | 3.37 | 2.99 | 3.17 | 5.55 | 5.07 | 5.47 | 6.42 | 9.45 | 5.11 | ||||||
| Vβ5.2 | 0.92 | 0.22 | 0.53 | 0.19 | 0.29 | 0.25 | 0.55 | 0.32 | 0.58 | ||||||
| Vβ5.3 | 2.17 | 2.42 | 2.25 | 0.49 | 0.38 | 0.5 | 0.15 | 0.14 | 0.2 | ||||||
| Vβ7.1 | 2.73 | 3.03 | 2.66 | 4.25 | 3.71 | 3.51 | 4.43 | 3.89 | 5.93 | ||||||
| Vβ7.2 | 0.04 | 0.06 | 0.01 | 1.11 | 0.7 | 0.85 | 0.32 | 0.28 | 0.4 | ||||||
| Vβ8 | 1.51 | 1.43 | 1.08 | 4.79 | 4.88 | 4.79 | 0.78 | 0.51 | 0.41 | ||||||
| Vβ9 | 2.05 | 1.39 | 3.1 | 0.82 | 0.67 | 0.49 | 0.9 | 0.87 | 0.57 | ||||||
| Vβ11 | 0.06 | 0.35 | 0.48 | 3.99 | 2.41 | 3.24 | 0.07 | 0.06 | 0.02 | ||||||
| Vβ12 | 0.51 | 1.14 | 0.58 | 3.55 | 3.97 | 3.11 | 0.7 | 0.96 | 0.38 | ||||||
| Vβ13.1 | 1.98 | 1.1 | 1.66 | 1.8 | 2.38 | 1.72 | 0.63 | 0.44 | 0.53 | ||||||
| Vβ13.2 | 8.52 | 1.53 | 13.24 | 1.83 | 1.49 | 1.66 | 1.78 | 1.24 | 1.26 | ||||||
| Vβ13.6 | 1.24 | 1.0 | 0.89 | 0.59 | 0.84 | 0.65 | 0.11 | 0.09 | 0.15 | ||||||
| Vβ14 | 13.97 | 19.4 | 17.73 | 5.93 | 7.48 | 4.97 | 6.73 | 8.55 | 6.45 | ||||||
| Vβ16 | 0.46 | 0.62 | 0.57 | 2.71 | 4.97 | 2.36 | 0.45 | 0.33 | 0.35 | ||||||
| Vβ17 | 0.38 | 0.25 | 0.33 | 2.65 | 1.78 | 1.6 | 0.13 | 0.35 | 0.21 | ||||||
| Vβ18 | 0.06 | 0.18 | 0.05 | 1.07 | 1.67 | 1.39 | 0.06 | 0.19 | 0.09 | ||||||
| Vβ20 | 0.5 | 0.39 | 0.47 | 1.25 | 1.28 | 0.75 | 0.46 | 0.41 | 0.28 | ||||||
| Vβ21.3 | 1.22 | 0.84 | 0.9 | 0.71 | 0.77 | 0.77 | 3.54 | 4.11 | 3.23 | ||||||
| Vβ22 | 3.19 | 2.12 | 2.9 | 1.78 | 1.9 | 1.97 | 1.33 | 0.81 | 0.9 | ||||||
| Vβ23 | 0.71 | 1.2 | 0.94 | 1.97 | 2.44 | 1.48 | 0.23 | 0.19 | 0.37 | ||||||
. | CTLs, donor 1 . | . | . | CTLs, donor 2 . | . | . | CTLs, donor 3 . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Vβ . | NT . | EV or CsiRNA . | SiRNA10 . | NT . | EV or CsiRNA . | SiRNA10 . | NT . | EV or CsiRNA . | SiRNA10 . | ||||||
| Vβ1 | 1.66 | 0.98 | 1.43 | 5.71 | 4.38 | 4.66 | 7.48 | 9.67 | 5.14 | ||||||
| Vβ2 | 1.54 | 1.81 | 1.54 | 4.55 | 4.59 | 4.59 | 8.05 | 7.61 | 8.24 | ||||||
| Vβ3 | 2.49 | 2.02 | 2.2 | 0.58 | 1.15 | 0.83 | 7.67 | 11.18 | 8.21 | ||||||
| Vβ4 | 0.01 | 0.06 | 0.06 | 0.11 | 0.32 | 0.16 | 0.03 | 0.06 | 0.02 | ||||||
| Vβ5.1 | 3.37 | 2.99 | 3.17 | 5.55 | 5.07 | 5.47 | 6.42 | 9.45 | 5.11 | ||||||
| Vβ5.2 | 0.92 | 0.22 | 0.53 | 0.19 | 0.29 | 0.25 | 0.55 | 0.32 | 0.58 | ||||||
| Vβ5.3 | 2.17 | 2.42 | 2.25 | 0.49 | 0.38 | 0.5 | 0.15 | 0.14 | 0.2 | ||||||
| Vβ7.1 | 2.73 | 3.03 | 2.66 | 4.25 | 3.71 | 3.51 | 4.43 | 3.89 | 5.93 | ||||||
| Vβ7.2 | 0.04 | 0.06 | 0.01 | 1.11 | 0.7 | 0.85 | 0.32 | 0.28 | 0.4 | ||||||
| Vβ8 | 1.51 | 1.43 | 1.08 | 4.79 | 4.88 | 4.79 | 0.78 | 0.51 | 0.41 | ||||||
| Vβ9 | 2.05 | 1.39 | 3.1 | 0.82 | 0.67 | 0.49 | 0.9 | 0.87 | 0.57 | ||||||
| Vβ11 | 0.06 | 0.35 | 0.48 | 3.99 | 2.41 | 3.24 | 0.07 | 0.06 | 0.02 | ||||||
| Vβ12 | 0.51 | 1.14 | 0.58 | 3.55 | 3.97 | 3.11 | 0.7 | 0.96 | 0.38 | ||||||
| Vβ13.1 | 1.98 | 1.1 | 1.66 | 1.8 | 2.38 | 1.72 | 0.63 | 0.44 | 0.53 | ||||||
| Vβ13.2 | 8.52 | 1.53 | 13.24 | 1.83 | 1.49 | 1.66 | 1.78 | 1.24 | 1.26 | ||||||
| Vβ13.6 | 1.24 | 1.0 | 0.89 | 0.59 | 0.84 | 0.65 | 0.11 | 0.09 | 0.15 | ||||||
| Vβ14 | 13.97 | 19.4 | 17.73 | 5.93 | 7.48 | 4.97 | 6.73 | 8.55 | 6.45 | ||||||
| Vβ16 | 0.46 | 0.62 | 0.57 | 2.71 | 4.97 | 2.36 | 0.45 | 0.33 | 0.35 | ||||||
| Vβ17 | 0.38 | 0.25 | 0.33 | 2.65 | 1.78 | 1.6 | 0.13 | 0.35 | 0.21 | ||||||
| Vβ18 | 0.06 | 0.18 | 0.05 | 1.07 | 1.67 | 1.39 | 0.06 | 0.19 | 0.09 | ||||||
| Vβ20 | 0.5 | 0.39 | 0.47 | 1.25 | 1.28 | 0.75 | 0.46 | 0.41 | 0.28 | ||||||
| Vβ21.3 | 1.22 | 0.84 | 0.9 | 0.71 | 0.77 | 0.77 | 3.54 | 4.11 | 3.23 | ||||||
| Vβ22 | 3.19 | 2.12 | 2.9 | 1.78 | 1.9 | 1.97 | 1.33 | 0.81 | 0.9 | ||||||
| Vβ23 | 0.71 | 1.2 | 0.94 | 1.97 | 2.44 | 1.48 | 0.23 | 0.19 | 0.37 | ||||||
Data represent the percentage of T cells recognized by the specific antibodies.
NT indicates nontransduced CTLs; EV, CTLs transduced with the pSUPER.puro empty; CsiRNA, CTLs transduced with pSUPER.puroCsiRNA; siRNA10, CTLs transduced with pSUPER.puro.siRNA10.