IFNγ release of CTLs transduced with siRNA10 and maintained in culture with or without CH-11 antibody
CTLs . | HLA*typing . | A2-CLG . | A2-GLC . | B8-RAK . | B8-QAK . | B35-AVL . | B35-HPV . | CTL alone . | A11†DEP . |
|---|---|---|---|---|---|---|---|---|---|
| 1 | A1,24;B8,18 | — | — | 543 ± 83‡ | 1199 ± 25 | — | — | 0 | NT |
| 1 + CH-11 | A1,24;B8,18 | — | — | 615 ± 64 | 1022 ± 185 | — | — | 0 | NT |
| 2 | A1,2,;B7,B8 | 499 ± 54 | 227 ± 21 | NT | NT | — | — | 0 | 0 |
| 2 + CH-11 | A1,2,;B7,B8 | 433 ± 2 | 296 ± 20 | NT | NT | — | — | 0 | 0 |
| 3 | A2,3;B35,57 | 208 ± 22 | NT | — | — | 310 ± 61 | 533 ± 87 | 0 | 0 |
| 3 + CH-11 | A2,3;B35,57 | 203 ± 34 | NT | — | — | 245 ± 76 | 553 ± 26 | 0 | 0 |
| 4 | A1,2;B7,8 | 12 ± 4 | NT | 338 ± 34 | 576 ± 100 | — | — | 0 | NT |
| 4 + CH-11 | A1,2;B7,8 | 32 ± 7 | NT | 326 ± 26 | 458 ± 50 | — | — | 0 | NT |
CTLs . | HLA*typing . | A2-CLG . | A2-GLC . | B8-RAK . | B8-QAK . | B35-AVL . | B35-HPV . | CTL alone . | A11†DEP . |
|---|---|---|---|---|---|---|---|---|---|
| 1 | A1,24;B8,18 | — | — | 543 ± 83‡ | 1199 ± 25 | — | — | 0 | NT |
| 1 + CH-11 | A1,24;B8,18 | — | — | 615 ± 64 | 1022 ± 185 | — | — | 0 | NT |
| 2 | A1,2,;B7,B8 | 499 ± 54 | 227 ± 21 | NT | NT | — | — | 0 | 0 |
| 2 + CH-11 | A1,2,;B7,B8 | 433 ± 2 | 296 ± 20 | NT | NT | — | — | 0 | 0 |
| 3 | A2,3;B35,57 | 208 ± 22 | NT | — | — | 310 ± 61 | 533 ± 87 | 0 | 0 |
| 3 + CH-11 | A2,3;B35,57 | 203 ± 34 | NT | — | — | 245 ± 76 | 553 ± 26 | 0 | 0 |
| 4 | A1,2;B7,8 | 12 ± 4 | NT | 338 ± 34 | 576 ± 100 | — | — | 0 | NT |
| 4 + CH-11 | A1,2;B7,8 | 32 ± 7 | NT | 326 ± 26 | 458 ± 50 | — | — | 0 | NT |
NT indicates not tested; —, not applicable.
The following HLA class I-restricted peptides were used for stimulation in an ELISPOT assay: HLA-A2 LMP2 epitope CLGGLLTML (CLG), HLA-A2 BMLF1 epitope GLCTLVAML (GLC), HLA-B8 BZLF1 epitope RAKFQLL (RAK), HLA-B8 EBNA3A QAKWRLQTL (QAK), HLA-B35 EBNA3B epitope AVLLHEESL (AVL), and HLA-B35 EBNA1 epitope HPVGEADYF (HPV).
The A11-DEP peptide was used as irrelevant peptide.
Number of spot-forming cells/105CTL.