Table 1. Stress- and oligomycin-induced... | American Society of Hematology

Table 1.

Stress- and oligomycin-induced changes of gene expression in MOLT-3 cells

Probe set ID	Control cells	Oligomycin	Doxorubicin	Doxorubicin + oligomycin	Etoposide	Etoposide + oligomycin	Gene	Function/pathway
202284_s_at	14	8	196	18	203	47	p21	Inhibitor of cyclin-dependent kinase activity, p53-target³⁵
203725_at	30	41	202	185	324	249	GADD45A	GADD family members; stress and DNA damage response, p53 targets³⁷
218346_s_at	112	73	384	175	412	247	PA26	GADD family members; stress and DNA damage response, p53 targets³⁷
205780_at	110	136	435	159	479	160	BIK	Proapoptotic BH-3 only protein; p53 target³⁹
208478_s_at	54	68	91	55	117	55	BAX	Proapoptotic p53 target³⁹
207813_s_at	60	58	326	153	321	132	FDXR	Ferredoxin reductase, electron transport chain, p53 target³⁶
203409_at	168	273	593	310	816	532	DDB2	DNA damage-binding protein; DNA repair, p53 target³⁷
201739_at	20	83	117	237	50	447	SGK	Kinase activity; glucose metabolism, transcriptionally repressed by p53⁴⁰
201694_s_at	90	404	58	207	86	314	EGR1	Transcription factor; early stress response, transcriptionally repressed by p53⁴¹
201631_s_at	105	62	1543	602	783	552	IER3/PRG1	Early stress response; p53 and NF B/rel target⁷³
201502_s_at	479	453	1974	2064	718	1666	IKBA	NFκB inhibitor, NFκB target⁴²
202871_at	82	102	263	260	258	338	TRAF4	TNF receptor-associated factor; NF-κB survival signaling⁴³
209795_at	87	305	311	489	144	618	CD69	Signal transmitting receptor, NFκB-target⁴²
202672_s_at	97	825	642	1113	601	1504	ATF3	Transcription factor; stress response⁴⁶
218145_at	74	327	70	296	77	349	TRB3	Regulator of glucose homeostasis⁴⁴
209822_s_at	36	232	34	243	39	238	VLDLR	Lipoprotein receptor, fatty acid metabolism⁴⁴
212501_at	225	1713	312	2171	243	1629	CEBPb	Transcription factor, regulator of energy homeostasis⁴⁵
202581_s_at	202	63	233	90	118	55	HSP70B	Heat-shock protein
203478_at	1403	745	1294	667	1312	692	NDUFC1	NADH dehydrogenase, subunit C1
201746_at	572	491	443	473	456	519	p53	Tumor suppressor; proapoptotic cellular stress response

Probe set ID	Control cells	Oligomycin	Doxorubicin	Doxorubicin + oligomycin	Etoposide	Etoposide + oligomycin	Gene	Function/pathway
202284_s_at	14	8	196	18	203	47	p21	Inhibitor of cyclin-dependent kinase activity, p53-target³⁵
203725_at	30	41	202	185	324	249	GADD45A	GADD family members; stress and DNA damage response, p53 targets³⁷
218346_s_at	112	73	384	175	412	247	PA26	GADD family members; stress and DNA damage response, p53 targets³⁷
205780_at	110	136	435	159	479	160	BIK	Proapoptotic BH-3 only protein; p53 target³⁹
208478_s_at	54	68	91	55	117	55	BAX	Proapoptotic p53 target³⁹
207813_s_at	60	58	326	153	321	132	FDXR	Ferredoxin reductase, electron transport chain, p53 target³⁶
203409_at	168	273	593	310	816	532	DDB2	DNA damage-binding protein; DNA repair, p53 target³⁷
201739_at	20	83	117	237	50	447	SGK	Kinase activity; glucose metabolism, transcriptionally repressed by p53⁴⁰
201694_s_at	90	404	58	207	86	314	EGR1	Transcription factor; early stress response, transcriptionally repressed by p53⁴¹
201631_s_at	105	62	1543	602	783	552	IER3/PRG1	Early stress response; p53 and NF B/rel target⁷³
201502_s_at	479	453	1974	2064	718	1666	IKBA	NFκB inhibitor, NFκB target⁴²
202871_at	82	102	263	260	258	338	TRAF4	TNF receptor-associated factor; NF-κB survival signaling⁴³
209795_at	87	305	311	489	144	618	CD69	Signal transmitting receptor, NFκB-target⁴²
202672_s_at	97	825	642	1113	601	1504	ATF3	Transcription factor; stress response⁴⁶
218145_at	74	327	70	296	77	349	TRB3	Regulator of glucose homeostasis⁴⁴
209822_s_at	36	232	34	243	39	238	VLDLR	Lipoprotein receptor, fatty acid metabolism⁴⁴
212501_at	225	1713	312	2171	243	1629	CEBPb	Transcription factor, regulator of energy homeostasis⁴⁵
202581_s_at	202	63	233	90	118	55	HSP70B	Heat-shock protein
203478_at	1403	745	1294	667	1312	692	NDUFC1	NADH dehydrogenase, subunit C1
201746_at	572	491	443	473	456	519	p53	Tumor suppressor; proapoptotic cellular stress response

MOLT-3 cells were treated with etoposide (1 μM) or doxorubicin (1 μM) in the presence or absence of oligomycin (10 μg/mL) for 3 hours and mRNA expression levels (quantified in fluorescence intensity units) were assessed using Affymetrix HG Focus Arrays. Control cells were investigated by 2 independent preparations, and mean gene expression values are given.

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