Representative characteristics of leukemias induced by populations of progenitors immortalized by Hoxa9
. | Peripheral blood cell analysis* . | . | . | . | . | . | . | . | FACS analysis, % positive . | . | . | . | . | . | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | . | . | . | . | . | Tissue mass, mg† . | . | . | . | Marrow . | . | . | Spleen . | . | |||||||||
| Mouse ID . | WBC, 103/μL . | Mye, % . | Lym, % . | Plat, 103/μL . | Hemat, % . | Spleen . | Thy . | Node . | Macl . | B220 . | Thy1.2 . | Macl . | B220 . | Thy1.2 . | |||||||||
| Hoxa9 plus Meis1 and SCF or SCF + IL-7 | |||||||||||||||||||||||
| Scf no. 1.1 | 91 | 91 | 7 | 370 | 40 | 138 | 14 | 6 | 88 | 3 | 1 | 62 | 6 | 12 | |||||||||
| Scf no. 1.2 | 91 | 86 | 13 | 405 | 46 | 450 | 29 | 19 | 94 | 2 | 1 | 73 | 11 | 14 | |||||||||
| Scf no. 2.1 | 76 | 97 | 3 | 627 | 35 | 365 | 35 | 14 | 84 | 5 | 1 | 72 | 10 | 9 | |||||||||
| Scf no. 2.2 | 68 | 88 | 12 | 331 | 40 | 236 | 27 | 15 | 96 | 4 | 1 | 66 | 16 | 20 | |||||||||
| Scf no. 3.1 | 140 | 91 | 9 | 771 | 26 | 350 | 32 | 17 | 72 | 5 | 0 | 74 | 9 | 10 | |||||||||
| Hoxa9 plus Meis1 and FL | |||||||||||||||||||||||
| Fl no. 1.1 | 153 | 93 | 6 | 531 | 35 | 400 | 21 | 21 | 85 | 3 | 1 | 66 | 5 | 4 | |||||||||
| Fl no. 2.1 | 120 | 79 | 16 | 406 | 27 | 664 | 29 | 12 | 55 | 62 | 1 | 72 | 28 | 7 | |||||||||
| Fl no. 3.1 | 105 | 94 | 4 | 461 | 36 | 433 | 27 | 15 | 70 | 30 | 0 | 60 | 8 | 10 | |||||||||
| Fl no. 3.2 | 115 | 98 | 2 | 737 | 36 | 421 | 19 | 22 | 95 | 1 | 3 | 70 | 8 | 16 | |||||||||
. | Peripheral blood cell analysis* . | . | . | . | . | . | . | . | FACS analysis, % positive . | . | . | . | . | . | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | . | . | . | . | . | Tissue mass, mg† . | . | . | . | Marrow . | . | . | Spleen . | . | |||||||||
| Mouse ID . | WBC, 103/μL . | Mye, % . | Lym, % . | Plat, 103/μL . | Hemat, % . | Spleen . | Thy . | Node . | Macl . | B220 . | Thy1.2 . | Macl . | B220 . | Thy1.2 . | |||||||||
| Hoxa9 plus Meis1 and SCF or SCF + IL-7 | |||||||||||||||||||||||
| Scf no. 1.1 | 91 | 91 | 7 | 370 | 40 | 138 | 14 | 6 | 88 | 3 | 1 | 62 | 6 | 12 | |||||||||
| Scf no. 1.2 | 91 | 86 | 13 | 405 | 46 | 450 | 29 | 19 | 94 | 2 | 1 | 73 | 11 | 14 | |||||||||
| Scf no. 2.1 | 76 | 97 | 3 | 627 | 35 | 365 | 35 | 14 | 84 | 5 | 1 | 72 | 10 | 9 | |||||||||
| Scf no. 2.2 | 68 | 88 | 12 | 331 | 40 | 236 | 27 | 15 | 96 | 4 | 1 | 66 | 16 | 20 | |||||||||
| Scf no. 3.1 | 140 | 91 | 9 | 771 | 26 | 350 | 32 | 17 | 72 | 5 | 0 | 74 | 9 | 10 | |||||||||
| Hoxa9 plus Meis1 and FL | |||||||||||||||||||||||
| Fl no. 1.1 | 153 | 93 | 6 | 531 | 35 | 400 | 21 | 21 | 85 | 3 | 1 | 66 | 5 | 4 | |||||||||
| Fl no. 2.1 | 120 | 79 | 16 | 406 | 27 | 664 | 29 | 12 | 55 | 62 | 1 | 72 | 28 | 7 | |||||||||
| Fl no. 3.1 | 105 | 94 | 4 | 461 | 36 | 433 | 27 | 15 | 70 | 30 | 0 | 60 | 8 | 10 | |||||||||
| Fl no. 3.2 | 115 | 98 | 2 | 737 | 36 | 421 | 19 | 22 | 95 | 1 | 3 | 70 | 8 | 16 | |||||||||
WBC indicates white blood cells; Mye, myeloid (principally progenitors in leukemic samples); Lym, B and T lymphocytes; Plat, platelets; Hemat, hematocrit; Thy, thymus; Node, average mass of each poplityl lymph node.
Average values from 4 normal mice were as follows: WBC, 3 × 103/μL; myeloid progenitors, 25%; platelets, 817 × 103/μL; and hematocrit, 45%
Average values from 4 normal mice were as follows: spleen, 106 mg; thymus, 29 mg; and node, <1 mg