Genomic signature of progenitors immortalized by Hoxa9 versus Hoxa9 plus Meis1
Gene . | A9/M . | A9 . | Δ no. . | Genebank ID . | Comments . |
|---|---|---|---|---|---|
| Increased expression in Hoxa9 plus Meis1-immortalized progenitors | |||||
| Cd34 | 3600 | A | > 50 | NM_133654 | Adhesion, homing, HSC marker |
| MD-1 | 3000 | A | > 50 | NM_010745 | Lymphoid Toll coreceptor |
| Flt3 | 2167 | A | > 50 | NM_010229 | Receptor tyrosine kinase, HSC marker |
| CD45-AP | 1045 | A | > 50 | NM_016933 | Activates CD45 tyrosine phosphatase |
| Itgb7 | 558 | A | > 50 | NM_013566 | Integrin beta 7, homing |
| Kcna3 | 501 | A | > 50 | A1323624 | Voltage gated K channel |
| Cd27 | 493 | A | > 50 | L24495 | TNF receptor 7, HSC marker |
| Erg1* | 465 | A | > 50 | AB073078 | Ets-related gene |
| Ngn | 450 | A | > 50 | AK002933 | Neurogranin |
| Ednra | 373 | A | > 50 | BC008277 | Endothelin receptor A, inhibits migration |
| IL7R | 350 | A | > 50 | NM_008372 | Interleukin 7 receptor |
| Gpr56 | 323 | A | > 50 | NM_018882 | G protein coupled receptor, Enriched in HSCs |
| CXXC5* | 300 | A | > 50 | AK015150 | Cystein methylation, transcription |
| Oaf | 260 | A | > 50 | BC025514 | Out at first, early development |
| RIP140* | 250 | A | > 50 | BB764550 | Nuclear receptor corepressor |
| Tspan2 | 180 | A | > 50 | AV234585 | Tetraspanin 2 |
| CD244 | 142 | A | > 50 | NM_018729 | NK cell receptor 2B4 |
| Gem | 103 | A | > 50 | U10551 | Ras family GTPase, HSC enriched |
| Ebf1* | 59 | A | > 50 | NM_007897 | Early B-cell factor |
| PLZF* | 50 | A | > 50 | AA419994 | Promyelocytic leukemia zinc finger |
| Meis* | 47 | A | > 50 | NM_010789 | Oncogene expressed by retrovirus |
| CRLR | 463 | 18 | 26 | AF209905 | Calcitonin rec.-related, HSC marker |
| Satb1* | 1300 | 90 | 14 | NM_009122 | Nuclear matrix attachment, DNA-binding |
| Tilz1b* | 1506 | 175 | 9 | AF201285 | Leucine zipper transcription factor |
| C1qR1 | 3025 | 430 | 7 | AF081789 | Complement rec. AA4.1, HSC marker |
| Tmsb10 | 3200 | 500 | 6 | NM_025284 | Thymosin beta 10 |
| Increased expression in Hoxa9-immortalized progenitors | |||||
| Slfn4 | A | 1963 | > 50 | AF099975 | Schlafen 4, growth regulatory gene |
| Tnfsf13 | A | 977 | > 50 | NM_023517 | TNF superfamily, member 13 |
| TGFRb3 | A | 623 | > 50 | BM122301 | Transforming growth factor rec. |
| Rnf128 | A | 493 | > 50 | NM_023270 | Ring finger protein 128: E3 ligase |
| ABCa13 | A | 467 | > 50 | BB277120 | ATP-binding cassette trans. A13 |
| Rhol | A | 393 | > 50 | AF309564 | GTPase, cytoskeletal org. |
| NB-I | A | 386 | > 50 | BC027283 | NB-1 glycoprotein |
| Pde2a | A | 230 | > 50 | BG069616 | Phosphodiesterase 2a |
| TLR4 | A | 208 | > 50 | NM_021297 | Toll-like receptor 4 |
| B4Ga1T6 | 95 | 2070 | 22 | BI685536 | Galactosyl transferase |
| Hsd11b1 | 97 | 1122 | 12 | NM_008288 | Hydroxysteroid 11 beta dehydro 1 |
| Tsp1 | 272 | 2747 | 10 | AV026492 | Thrombospondin-1, ECM protein |
| Tfec* | 317 | 3200 | 10 | NM_031198 | Myeloid Myc-related bHLH TF |
| Sortilin-1 | 31 | 311 | 10 | NM_019972 | Trafficking/sorting |
| ALAS1 | 437 | 3770 | 9 | BC022110 | Amino levulinic acid synthetase |
| Clecsf12 | 167 | 1500 | 9 | AF262985 | C-type lectin superfamily 12 |
| Catns | 20 | 158 | 8 | NM_007615 | p120 catenin |
| Csf2ra | 161 | 1229 | 8 | NM_009970 | GM-CSF receptor, alpha chain |
| Bscl2 | 80 | 602 | 8 | BB223872 | Fat metabolism |
| DORA | 230 | 1673 | 7 | NM_030691 | Ig superfamily, member 6 |
| Ms4a3 | 843 | 5533 | 7 | NM_133246 | Hematospecific membrane protein |
| Akr1cl3 | 127 | 782 | 6 | BC021937 | Aldol Keto reductase c13 |
| NE | 1333 | 7800 | 6 | NM_015779 | Neutrophil elastase |
| Atip1 | 73 | 402 | 6 | AW554551 | Inhibits tyrosine kinases |
| Gda | 620 | 2820 | 5 | AF174583 | Guanine deaminase |
| Gng31g | 118 | 603 | 5 | BB223872 | G protein gamma 3 linked |
| Controls | |||||
| Cyclin A2 | 1180 | 1470 | — | NM_009829 | Binds cdk2, G1/S cell cycle regulation |
| Cyclin B | 670 | 657 | — | X58708 | Binds cdk1, G2/M cell cycle regulation |
| Mpo | 10750 | 9300 | — | NM_010824 | Myeloperoxidase |
| Myb | 4900 | 4800 | — | NM_033597 | Hematopoietic transcription factor |
| Bmi-1 | 257 | 343 | — | M64279 | Maintains HSC self-renewal |
| Mll | 96 | 88 | — | AK017541 | Histone methyltransferase |
| Prtn3 | 7000 | 8030 | — | NM_011178 | Myeloblastin, pre-pro-proteinase 3 |
Gene . | A9/M . | A9 . | Δ no. . | Genebank ID . | Comments . |
|---|---|---|---|---|---|
| Increased expression in Hoxa9 plus Meis1-immortalized progenitors | |||||
| Cd34 | 3600 | A | > 50 | NM_133654 | Adhesion, homing, HSC marker |
| MD-1 | 3000 | A | > 50 | NM_010745 | Lymphoid Toll coreceptor |
| Flt3 | 2167 | A | > 50 | NM_010229 | Receptor tyrosine kinase, HSC marker |
| CD45-AP | 1045 | A | > 50 | NM_016933 | Activates CD45 tyrosine phosphatase |
| Itgb7 | 558 | A | > 50 | NM_013566 | Integrin beta 7, homing |
| Kcna3 | 501 | A | > 50 | A1323624 | Voltage gated K channel |
| Cd27 | 493 | A | > 50 | L24495 | TNF receptor 7, HSC marker |
| Erg1* | 465 | A | > 50 | AB073078 | Ets-related gene |
| Ngn | 450 | A | > 50 | AK002933 | Neurogranin |
| Ednra | 373 | A | > 50 | BC008277 | Endothelin receptor A, inhibits migration |
| IL7R | 350 | A | > 50 | NM_008372 | Interleukin 7 receptor |
| Gpr56 | 323 | A | > 50 | NM_018882 | G protein coupled receptor, Enriched in HSCs |
| CXXC5* | 300 | A | > 50 | AK015150 | Cystein methylation, transcription |
| Oaf | 260 | A | > 50 | BC025514 | Out at first, early development |
| RIP140* | 250 | A | > 50 | BB764550 | Nuclear receptor corepressor |
| Tspan2 | 180 | A | > 50 | AV234585 | Tetraspanin 2 |
| CD244 | 142 | A | > 50 | NM_018729 | NK cell receptor 2B4 |
| Gem | 103 | A | > 50 | U10551 | Ras family GTPase, HSC enriched |
| Ebf1* | 59 | A | > 50 | NM_007897 | Early B-cell factor |
| PLZF* | 50 | A | > 50 | AA419994 | Promyelocytic leukemia zinc finger |
| Meis* | 47 | A | > 50 | NM_010789 | Oncogene expressed by retrovirus |
| CRLR | 463 | 18 | 26 | AF209905 | Calcitonin rec.-related, HSC marker |
| Satb1* | 1300 | 90 | 14 | NM_009122 | Nuclear matrix attachment, DNA-binding |
| Tilz1b* | 1506 | 175 | 9 | AF201285 | Leucine zipper transcription factor |
| C1qR1 | 3025 | 430 | 7 | AF081789 | Complement rec. AA4.1, HSC marker |
| Tmsb10 | 3200 | 500 | 6 | NM_025284 | Thymosin beta 10 |
| Increased expression in Hoxa9-immortalized progenitors | |||||
| Slfn4 | A | 1963 | > 50 | AF099975 | Schlafen 4, growth regulatory gene |
| Tnfsf13 | A | 977 | > 50 | NM_023517 | TNF superfamily, member 13 |
| TGFRb3 | A | 623 | > 50 | BM122301 | Transforming growth factor rec. |
| Rnf128 | A | 493 | > 50 | NM_023270 | Ring finger protein 128: E3 ligase |
| ABCa13 | A | 467 | > 50 | BB277120 | ATP-binding cassette trans. A13 |
| Rhol | A | 393 | > 50 | AF309564 | GTPase, cytoskeletal org. |
| NB-I | A | 386 | > 50 | BC027283 | NB-1 glycoprotein |
| Pde2a | A | 230 | > 50 | BG069616 | Phosphodiesterase 2a |
| TLR4 | A | 208 | > 50 | NM_021297 | Toll-like receptor 4 |
| B4Ga1T6 | 95 | 2070 | 22 | BI685536 | Galactosyl transferase |
| Hsd11b1 | 97 | 1122 | 12 | NM_008288 | Hydroxysteroid 11 beta dehydro 1 |
| Tsp1 | 272 | 2747 | 10 | AV026492 | Thrombospondin-1, ECM protein |
| Tfec* | 317 | 3200 | 10 | NM_031198 | Myeloid Myc-related bHLH TF |
| Sortilin-1 | 31 | 311 | 10 | NM_019972 | Trafficking/sorting |
| ALAS1 | 437 | 3770 | 9 | BC022110 | Amino levulinic acid synthetase |
| Clecsf12 | 167 | 1500 | 9 | AF262985 | C-type lectin superfamily 12 |
| Catns | 20 | 158 | 8 | NM_007615 | p120 catenin |
| Csf2ra | 161 | 1229 | 8 | NM_009970 | GM-CSF receptor, alpha chain |
| Bscl2 | 80 | 602 | 8 | BB223872 | Fat metabolism |
| DORA | 230 | 1673 | 7 | NM_030691 | Ig superfamily, member 6 |
| Ms4a3 | 843 | 5533 | 7 | NM_133246 | Hematospecific membrane protein |
| Akr1cl3 | 127 | 782 | 6 | BC021937 | Aldol Keto reductase c13 |
| NE | 1333 | 7800 | 6 | NM_015779 | Neutrophil elastase |
| Atip1 | 73 | 402 | 6 | AW554551 | Inhibits tyrosine kinases |
| Gda | 620 | 2820 | 5 | AF174583 | Guanine deaminase |
| Gng31g | 118 | 603 | 5 | BB223872 | G protein gamma 3 linked |
| Controls | |||||
| Cyclin A2 | 1180 | 1470 | — | NM_009829 | Binds cdk2, G1/S cell cycle regulation |
| Cyclin B | 670 | 657 | — | X58708 | Binds cdk1, G2/M cell cycle regulation |
| Mpo | 10750 | 9300 | — | NM_010824 | Myeloperoxidase |
| Myb | 4900 | 4800 | — | NM_033597 | Hematopoietic transcription factor |
| Bmi-1 | 257 | 343 | — | M64279 | Maintains HSC self-renewal |
| Mll | 96 | 88 | — | AK017541 | Histone methyltransferase |
| Prtn3 | 7000 | 8030 | — | NM_011178 | Myeloblastin, pre-pro-proteinase 3 |
Hematopoietic stem cell markers are underlined. — indicates no significant change.
Transcription factor genes