Real-time quantitative RT-PCR determination of IL-4 transcript levels
. | Days ACT . | . | . | ||
|---|---|---|---|---|---|
| Cell donors . | 5-8 . | 12-19 . | 28-34 . | ||
| Skin | |||||
| WT C57BL/6 | 1.83 ± 4.20 | 1.80 ± 1.01 | 1.38 ± 1.70 | ||
| CCR6-/- C57BL/6 | 0.97 ± 1.10 | 3.37 ± 1.41 | 1.37 ± 1.23 | ||
| WT BALB/c | ND | 52.00 ± 1.08 | 32.00 ± 6.30 | ||
| Small intestine | |||||
| WT C57BL/6 | 1.02 ± 4.20 | 1.93 ± 1.04 | 1.00 ± 0.10 | ||
| CCR6-/- C57BL/6 | 1.70 ± 1.30 | 1.88 ± 1.60 | 1.21 ± 2.43 | ||
| WT BALB/c | ND | 16.00 ± 4.00 | 8.00 ± 1.95 | ||
. | Days ACT . | . | . | ||
|---|---|---|---|---|---|
| Cell donors . | 5-8 . | 12-19 . | 28-34 . | ||
| Skin | |||||
| WT C57BL/6 | 1.83 ± 4.20 | 1.80 ± 1.01 | 1.38 ± 1.70 | ||
| CCR6-/- C57BL/6 | 0.97 ± 1.10 | 3.37 ± 1.41 | 1.37 ± 1.23 | ||
| WT BALB/c | ND | 52.00 ± 1.08 | 32.00 ± 6.30 | ||
| Small intestine | |||||
| WT C57BL/6 | 1.02 ± 4.20 | 1.93 ± 1.04 | 1.00 ± 0.10 | ||
| CCR6-/- C57BL/6 | 1.70 ± 1.30 | 1.88 ± 1.60 | 1.21 ± 2.43 | ||
| WT BALB/c | ND | 16.00 ± 4.00 | 8.00 ± 1.95 | ||
Results shown represent x-fold increase in IL-4 mRNA levels (experimental vs control).
ND indicates not determined.