Table 1. UCB cell fractionation . SSC lo... | American Society of Hematology

Table 1.

UCB cell fractionation

	SSC^lo ALDH^br	lin^neg SSC^lo CD34⁺	lin^neg SSC^lo ALDH^brCD34⁺	lin^neg SSC^lo ALDH^negCD34⁺	lin^neg SSC^lo ALDH^brCD34^neg
Total UCB, %
Mean ± SD	0.96 ± 0.5	0.90 ± 0.63	0.48 ± 0.29	0.27 ± 0.31	0.25 ± 0.26
Median	0.91	0.8	0.46	0.17	0.16
Mean CD34 fluorescence
Mean ± SD	—	320.7 ± 154	391.3 ± 198.8	188.4 ± 107.5	5.69 ± 1.9
Median	—	305.5	369.7	146.4	5.46

	SSC^lo ALDH^br	lin^neg SSC^lo CD34⁺	lin^neg SSC^lo ALDH^brCD34⁺	lin^neg SSC^lo ALDH^negCD34⁺	lin^neg SSC^lo ALDH^brCD34^neg
Total UCB, %
Mean ± SD	0.96 ± 0.5	0.90 ± 0.63	0.48 ± 0.29	0.27 ± 0.31	0.25 ± 0.26
Median	0.91	0.8	0.46	0.17	0.16
Mean CD34 fluorescence
Mean ± SD	—	320.7 ± 154	391.3 ± 198.8	188.4 ± 107.5	5.69 ± 1.9
Median	—	305.5	369.7	146.4	5.46

UCB cells were fractionated as depicted in Figure 1. Mean (± SD) and median percentages were calculated for lin^neg SSC^lo ALDH^br CD34⁺, lin^neg SSC^lo ALDH^neg CD34⁺, and lin^neg SSC^lo ALDH^br CD34^neg cell fractions (n = 25). Mean (± SD) and median mean fluorescence intensity (MFI) for CD34 expression were calculated for each cell fraction (n = 25). The intensity of CD34 expression was significantly different between the ALDH^br CD34⁺ and the ALDH^neg CD34⁺ cell subsets (P < .0001). See also Figure 1F-G.

— indicates not determined.

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