Table 2. Cell development in LTC . lin... | American Society of Hematology

Table 2.

Cell development in LTC

	lin^neg CD34⁺	ALDH^br CD34⁺	ALDH^neg CD34⁺	P
Cell expansion in primary LTC, -fold
Expansion*				.0625
Mean ± SD	4083 ± 1922	3664 ± 2120	827 ± 1142
Median	3479	3350	285
Myeloid CFUs/100 initiating cells
Primary LTC†				.004
Mean ± SD	260.9 ± 159	309.2 ± 180	4.85 ± 10.4
Median	294.9	349.4	0
Secondary LTC†				.004
Mean ± SD	13.9 ± 8.5	54.6 ± 95.1	0.36 ± 0.56
Median	9.74	25.9	0

	lin^neg CD34⁺	ALDH^br CD34⁺	ALDH^neg CD34⁺	P
Cell expansion in primary LTC, -fold
Expansion*				.0625
Mean ± SD	4083 ± 1922	3664 ± 2120	827 ± 1142
Median	3479	3350	285
Myeloid CFUs/100 initiating cells
Primary LTC†				.004
Mean ± SD	260.9 ± 159	309.2 ± 180	4.85 ± 10.4
Median	294.9	349.4	0
Secondary LTC†				.004
Mean ± SD	13.9 ± 8.5	54.6 ± 95.1	0.36 ± 0.56
Median	9.74	25.9	0

LTCs were established with lin^neg SSC^lo CD34⁺, lin^neg SSC^lo ALDH^br CD34⁺, lin^neg SSC^lo ALDH^neg CD34⁺, and lin^neg SSC^lo ALDH^br CD34^neg cells, as described in “Materials and methods.” Fifty to 500 cells were used to initiate the primary LTC. In some cultures, the total expansion of CD45⁺ cells was estimated by flow cytometry after 5 weeks.

Lin^neg SSC^lo ALDH^br CD34^neg cells were not supported under these culture conditions. Statistical comparisons (P) were drawn between the lin^neg SSC^lo ALDH^br CD34⁺ and the lin^neg SSC^lo ALDH^neg CD34⁺ cell fractions using paired, 1-tailed nonparametric analyses (Wilcoxon signed rank test).

†

Mean (±SD) and median frequencies for myeloid CFUs were normalized per 100 cells that were used to initiate the culture.

View Large

This Feature Is Available To Subscribers Only