Fibromodulin expression in activated cells
Stimulator by cell type . | CD25+ cells, % . | CD69+ cells, % . | Fold increase of fibromodulin expression* . |
|---|---|---|---|
| Enriched normal B cells, purity >90% | |||
| Unstimulated | 24 | 6 | 1 |
| DMEM alone | 32 | 17 | 12 |
| DMEM + CD40L | 74 | 88 | 1 |
| DMEM + PMA/ionomycin | 95 | 62 | 24 |
| Enriched normal T cells, purity >70% | |||
| Unstimulated | 22 | 3 | 1 |
| DMEM alone | 8 | 8 | 4 |
| DMEM + anti-CD3 | 73 | 65 | 2.5 |
| DMEM + anti-CD3 | ND | ND | 14 |
| Enriched tonsil B cells, purity >90% | |||
| Unstimulated | 4 | 54 | 1 |
| DMEM alone | 36 | 51 | 3 |
| DMEM + CD40L | 75 | 65 | 1 |
| DMEM + PMA/ionomycin | 76 | 77 | 1 |
| B-CLL | |||
| Unstimulated | 48 | 21 | 1 |
| DMEM alone | 57 | 18 | 5 |
| DMEM + CD40L | 96 | 99 | 1 |
| DMEM + PMA/ionomycin | 100 | 30 | 1 |
| EHEB | |||
| RPMI medium alone† | 11 | 12 | 1 |
| RPMI medium alone | 17 | 14 | 1 |
| RPMI medium + CD40L | 21 | 15 | 1 |
| RPMI medium + PMA/ionomycin | 27 | 12 | 1 |
| DMEM alone | 13 | 26 | 12 |
| DMEM + PMA/ionomycin | ND | ND | 114 |
| I83-E95 | |||
| RPMI medium alone† | 3 | 5 | 1 |
| RPMI medium alone | 7 | 6 | 1 |
| RPMI medium + CD40L | 11 | 6 | 1 |
| RPMI medium + PMA/ionomycin | ND | ND | ND |
| DMEM alone | 3 | 3 | 17 |
| DMEM + PMA/ionomycin | 33 | 8 | 21 |
Stimulator by cell type . | CD25+ cells, % . | CD69+ cells, % . | Fold increase of fibromodulin expression* . |
|---|---|---|---|
| Enriched normal B cells, purity >90% | |||
| Unstimulated | 24 | 6 | 1 |
| DMEM alone | 32 | 17 | 12 |
| DMEM + CD40L | 74 | 88 | 1 |
| DMEM + PMA/ionomycin | 95 | 62 | 24 |
| Enriched normal T cells, purity >70% | |||
| Unstimulated | 22 | 3 | 1 |
| DMEM alone | 8 | 8 | 4 |
| DMEM + anti-CD3 | 73 | 65 | 2.5 |
| DMEM + anti-CD3 | ND | ND | 14 |
| Enriched tonsil B cells, purity >90% | |||
| Unstimulated | 4 | 54 | 1 |
| DMEM alone | 36 | 51 | 3 |
| DMEM + CD40L | 75 | 65 | 1 |
| DMEM + PMA/ionomycin | 76 | 77 | 1 |
| B-CLL | |||
| Unstimulated | 48 | 21 | 1 |
| DMEM alone | 57 | 18 | 5 |
| DMEM + CD40L | 96 | 99 | 1 |
| DMEM + PMA/ionomycin | 100 | 30 | 1 |
| EHEB | |||
| RPMI medium alone† | 11 | 12 | 1 |
| RPMI medium alone | 17 | 14 | 1 |
| RPMI medium + CD40L | 21 | 15 | 1 |
| RPMI medium + PMA/ionomycin | 27 | 12 | 1 |
| DMEM alone | 13 | 26 | 12 |
| DMEM + PMA/ionomycin | ND | ND | 114 |
| I83-E95 | |||
| RPMI medium alone† | 3 | 5 | 1 |
| RPMI medium alone | 7 | 6 | 1 |
| RPMI medium + CD40L | 11 | 6 | 1 |
| RPMI medium + PMA/ionomycin | ND | ND | ND |
| DMEM alone | 3 | 3 | 17 |
| DMEM + PMA/ionomycin | 33 | 8 | 21 |
Fibromodulin expression was determined using real-time quantitative PCR. Cells were stimulated for 48 hours unless otherwise marked.
ND indicates not determined.
Results are expressed as fold increase compared with unstimulated cells at time 0 h. Values are the mean of 2 experiments.
Cells were stimulated for 0 hours.